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Validation of a miniaturised near infrared spectrometer for contaminant assessment in biodiesel

2023Journal article Open access
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dc.contributor.authorMonteiro, Luísa
dc.contributor.authorZoio, Paulo
dc.contributor.authorCarvalho, M. B. P.
dc.contributor.authorFonseca, Luís P. P.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2024-03-22T16:47:26Z
dc.date.available2024-03-22T16:47:26Z
dc.date.issued2023
dc.description.abstractA new method to simultaneously predict cells viability, proliferation and metabolic status, in a rapid, simple but also specific and sensitive mode was developed. The method is based on mid-infrared (MIR) spectroscopic analysis of cells. As model system were used Human embryonic kidney (HEK) 293 cells and T lymphocytes. After submitting cells to different environments as the toxic dimethyl sulfoxide, or metabolic activation, cells viability was analyzed by optical microscopy after coloration with trypan blue, and the cell count was determined with a Neubauer hemocytometer. The principal component analysis (PCA) of the cells second derivative spectra enabled to discriminate the cells viability and the cells proliferation as assayed by conventional methods, while spectra PCA and Hierarchical Cluster Analysis (HCA) enabled to discriminate T cells metabolic activation. The new methods, based on MIR spectroscopy, present the advantages of being applicable in automatic, simple and high-throughput mode in relation to the conventional methods.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi.org/10.17758/EIRAI19.F0623108pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/17203
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInternational Forum of Engineers & Practitionerspt_PT
dc.relationLA/P/0140/2020pt_PT
dc.relationNeproMD/ISEL/2020pt_PT
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Bioengineering and Biosciences
dc.relation.publisherversionhttps://fenp.org/procceeding.php/39pt_PT
dc.subjectbiodieselpt_PT
dc.subjectportable NIR spectrometerpt_PT
dc.subjectcontaminantspt_PT
dc.subjectadulterationpt_PT
dc.subjectFTIR spectroscopypt_PT
dc.titleValidation of a miniaturised near infrared spectrometer for contaminant assessment in biodieselpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT
oaire.citation.endPage2pt_PT
oaire.citation.startPage1pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameMonteiro
person.familyNameMartins Zoio
person.familyNameCarvalho
person.familyNameP. Fonseca
person.familyNameCalado
person.givenNameLuísa
person.givenNamePaulo Jorge
person.givenNameMarcela
person.givenNameLuis
person.givenNameCecília
person.identifier2733108
person.identifier130332
person.identifier.ciencia-id7516-3623-FE91
person.identifier.ciencia-id951D-DF38-3397
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0001-8562-0644
person.identifier.orcid0000-0003-4295-9271
person.identifier.orcid0000-0002-3445-3871
person.identifier.orcid0000-0001-8429-6977
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridA-4228-2013
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id55916288400
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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