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Development of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strain

2003Journal article Restricted access
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dc.contributor.authorCalado, Cecília
dc.contributor.authorAlmeida, Claúdio
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorFonseca, Luís P. P.
dc.date.accessioned2020-10-02T09:10:47Z
dc.date.available2020-10-02T09:10:47Z
dc.date.issued2003
dc.description.abstractSaccharomyces cerevisiae SU50 strain was cultivated with different concentrations of glucose and galactose with the aim of increasing cutinase activity, cutinase yield on the carbon source, and bioreactor productivity. Cultivations in shake flasks with galactose as the sole carbon source, with sugar concentrations between 10 and 40 g/l, exhibited growth-associated cutinase production and a constant specificity of cutinase secretion. Furthermore, as the galactose concentration increased to values higher than 15 g/l, a progressively higher maximum specific galactose consumption rate and a consequent higher alcoholic fermentation occurred, resulting in progressively lower biomass yields on the carbon source and cutinase yields on biomass. Using high glucose and galactose concentrations in a well-aerated bioreactor resulted in a high biomass productivity (0.5 g dcw/l/h), a high cutinase yield on biomass (21.5 U/mg dew), a final high cutinase secretion efficiency (97%), and plasmid stability (99%). Based on these studies, a two phase fed-batch cultivation strategy was developed. A batch phase with high glucose and galactose concentrations, followed by a fedbatch with a constant feed rate with galactose as the sole carbon source in order to minimize the repression of the GAL 7 promoter, were established. The feed rate was estimated to maintain a pre-determined concentration of galactose (20 g/l) on the culture medium in order to maximize the efficiency of cutinase secretion and minimize the galactose alcoholic fermentation. By this cultivation strategy, enhancements of 3.6-fold in cutinase activity, 1.2-fold in cutinase yield on the carbon source, and 8.7-fold culture productivity were obtained in relation to a batch cultivation performed in shake flasks with 20 g/l of galactose.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCALADO, Cecília R. C.; [et al] – Development of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strain. Journal of Bioscience and Bioengineering. ISSN 1389-1723. Vol. 96, N.º 2 (2003), pp. 141-148pt_PT
dc.identifier.doi10.1016/S1389-1723(03)90116-2pt_PT
dc.identifier.issn1389-1723
dc.identifier.urihttp://hdl.handle.net/10400.21/12268
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationPRAXIS XXI program, Ministério da Ciência e Tecnologia, Portugal (BD/l8276/98 and BM/l9093/99)pt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S1389172303901162?token=E5B8E2EBD30994116E515EAC858B91A8B0B497B5F036919EBF23418808DEFC2F2A13D38BB5A4C3FF773D03A2453FD8A0pt_PT
dc.subjectCutinasept_PT
dc.subjectRecombinantpt_PT
dc.subjectSaccharomyces cerevisiaept_PT
dc.subjectCultivation strategypt_PT
dc.titleDevelopment of a fed-batch cultivation strategy for the enhanced production and secretion of cutinase by a recombinant Saccharomyces cerevisiae SU50 strainpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage148pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage141pt_PT
oaire.citation.titleJournal of Bioscience and Bioengineeringpt_PT
oaire.citation.volume96pt_PT
person.familyNameCalado
person.familyNameCabral
person.familyNameP. Fonseca
person.givenNameCecília
person.givenNameJoaquim M.S.
person.givenNameLuis
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.ciencia-id951D-DF38-3397
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.orcid0000-0001-8429-6977
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.ridA-4228-2013
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
person.identifier.scopus-author-id55916288400
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication596986f6-6f41-4107-a097-bc205ecdb95a
relation.isAuthorOfPublication5c533551-5113-4c0a-93f1-9c50cbd796bc
relation.isAuthorOfPublication.latestForDiscovery5c533551-5113-4c0a-93f1-9c50cbd796bc

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