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An approach to quality control of the dithiothreitol (DTT) assay for the determination of oxidative potential of atmospheric particulate matter

2026-01Artigo científico Acesso aberto
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorVicente, Carolina
dc.contributor.authorGonçalves, Sara
dc.contributor.authorGamelas, Carla
dc.contributor.authorAlmeida, Susana Marta
dc.contributor.authorCanha, Nuno
dc.date.accessioned2026-04-14T09:32:38Z
dc.date.available2026-04-14T09:32:38Z
dc.date.issued2026-01
dc.descriptionThis research was funded by the Portuguese Foundation for Science and Technology (FCT, Portugal), through the PhD fellowship PRT/BD/154533/2023, and the financial support to C2TN/IST.
dc.description.abstractThe oxidative potential (OP) of airborne particulate matter (PM) has emerged as a promising metric to assess the capacity of particles to induce oxidative stress and related health effects. Thus, ensuring the reliability and comparability of OP measurements is essential for accurate environmental and toxicological assessment. This study aimed to develop and evaluate a quality control approach for the dithiothreitol (DTT) assay used in OP determination. The DTT assay provides an estimation of how harmful PM can be to human health through oxidative stress, relating it to the consumption of DTT during the test period. Two experiments were conducted using the Standard Reference Material (SRM) 1648–Urban Particulate Matter (NIST, USA). The first assessed the effect of trichloroacetic acid (TCA) addition order and the feasibility of using SRM 1648 as a reference material. The second evaluated the stability of the SRM solution over 63 days. Statistical analysis (Mann–Whitney test) indicated that the order of TCA addition did not significantly affect OP values (p > 0.05). SRM 1648 solution determination showed high reproducibility (mean DTTₘ = 14.6 ± 2.4 pmol·min−1·µg−1), although a gradual increase in DTT metrics was observed over time, consistent with progressive dissolution. The results support the application of SRM 1648 as a reference material for DTT assay quality control, supporting methodological harmonization in OP determination, provided that a freshly prepared solution is used.eng
dc.identifier.citationVicente C, Gonçalves S, Gamelas C, Almeida SM, Canha N. An approach to quality control of the dithiothreitol (DTT) assay for the determination of oxidative potential of atmospheric particulate matter. Environments. 2026;13(1):6.
dc.identifier.doi10.3390/environments13010006
dc.identifier.issn2076-3298
dc.identifier.urihttp://hdl.handle.net/10400.21/22782
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI AG
dc.relationC2TN/IST (UID/04349/2025: https://doi.org/10.54499/UID/04349/2025)
dc.relation.hasversionhttps://www.mdpi.com/2076-3298/13/1/6
dc.relation.ispartofEnvironments
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectOxidative potential
dc.subjectParticulate matter
dc.subjectDithiothreitol
dc.subjectStability
dc.subjectQuality control
dc.titleAn approach to quality control of the dithiothreitol (DTT) assay for the determination of oxidative potential of atmospheric particulate mattereng
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1
oaire.citation.startPage6
oaire.citation.titleEnvironments
oaire.citation.volume13
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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