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Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?

2020-08Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorDias, Marta
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorAłtyn, Iwona
dc.contributor.authorViegas, Susana
dc.contributor.authorCaetano, Liliana Aranha
dc.date.accessioned2020-08-31T13:46:13Z
dc.date.available2020-08-31T13:46:13Z
dc.date.issued2020-08
dc.descriptionProject nº 23222 (02/SAICT/2016).pt_PT
dc.descriptionProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL).pt_PT
dc.description.abstractThe use of Filtering Respiratory Protective Devices (FRPD) is mandatory in Portugal to protect workers from the waste industry of harmful exposures. Deleterious health effects of exposure to bioburden via inhalation and/or ingestion include respiratory symptoms and nephrotoxicity. Between January and February 2019, 118 FRPD samples were collected in one waste sorting industry and characterized regarding microbial contamination and cytotoxicity, defined as cell metabolic activity, through the MTT colorimetric assay (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). Cytotoxic effect was classified according to the percentage of extinction values with respect to the control group, as follows: absent (≥90); low (80% to 90%, +); medium (60% to 79%, ++); and high (below 60%, +++). For 113 samples the MTT assay revealed a cytotoxic effect in A549 cells, of which 81 presented high cytotoxicity. In SK cells, a cytotoxic effect was observed in 56 samples, of which five displayed a high cytotoxic effect. Several moderate (p<0.05) to strong (p<0.01) correlations were found between higher bacterial and fungal counts both in interior layers (fungi and bacteria) and in exhalation valves (fungi) of FRPD samples and reduced cellular metabolic activity of SK cells. On the basis of the obtained results for the cytotoxic effect of FRPD samples on two different cells lines, it was determined that A549 cells exhibited a cytotoxic effect for a higher number of FRPD, whereas the SK cells model correlated better with the other assessed parameters, namely, bacterial and fungal load and conditions of FRPD use. Although the results are not conclusive on the most appropriate cell line to assess FRPD cytotoxicity, they reinforce the importance of in vitro toxicology in exposure assessments to determine the cytotoxicity of mixtures of contaminants, for better risk characterization and selection of appropriate risk management measures.pt_PT
dc.description.versioninfo:eu-repo/semantics/acceptedVersionpt_PT
dc.identifier.citationViegas C, Dias M, Almeida B, Carolino E, Viegas S, Caetano LA, et al. Cytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization? Environ Res. 2020;191:110134.pt_PT
dc.identifier.doi10.1016/j.envres.2020.110134pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12198
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”pt_PT
dc.relationProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0013935120310318?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectFiltering respiratory protective devicespt_PT
dc.subjectWaste sorting industrypt_PT
dc.subjectIn vitro modelspt_PT
dc.subjectCytotoxic potentialpt_PT
dc.subjectRisk characterizationpt_PT
dc.subjectRisk managementpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”pt_PT
dc.subjectProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleCytotoxic effect of filtering respiratory protective devices from the waste sorting industry: is in vitro toxicology useful for risk characterization?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage110134pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume191pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.familyNameAranha Caetano
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.givenNameLiliana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.orcid0000-0003-1496-2609
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery77930d39-ed34-44dc-a4a6-9bf833e5e688

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