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Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment

1999-09Journal article Open access
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dc.contributor.authorDomingues, Célia
dc.contributor.authorSoares, Helena
dc.contributor.authorRodrigues-Pousada, Claudina
dc.contributor.authorCyrne, Luisa
dc.date.accessioned2018-09-21T12:47:46Z
dc.date.available2018-09-21T12:47:46Z
dc.date.issued1999-09
dc.description.abstractWe report here the cloning and the characterization of the Tetrahymena pyriformis chaperonin-containing-TCP1 theta gene (TpCCTθ), an orthologue of the mouse chaperonin gene CCTθ. TpCCTθ gene is interrupted by eight introns, ranging in size between 91 and 419 nucleotides, and encodes a protein consisting of 540 amino acid residues (59.1 kDa), with a putative pI of 5.73. The amino acid sequence of TpCCTθ reveals 39.4–46.0% identity with the sequences of Candida albicans and mouse CCTθ subunits and 28.0–32.6% identity with the other TpCCT subunits known so far. We have studied the expression of this gene in exponentially growing Tetrahymena cells and in cells treated with colchicine for different times. The steady-state levels of CCTθ mRNA rapidly decrease in the first 30 min of colchicine treatment. Interestingly, treatment for subsequent 60 min gives expression levels higher than those found in exponentially growing cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDomingues C, Soares H, Rodrigues-Pousada C, Cyrne L. Structure of Tetrahymena CCTθ gene and its expression under colchicine treatment. Biochim Biophys Acta. 1999;1446(3):443-9.pt_PT
dc.identifier.doi10.1016/S0167-4781(99)00111-6pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/8877
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0167478199001116pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCCTθ-chaperonin genept_PT
dc.subjectCiliated protozoanpt_PT
dc.subjectGene expressionpt_PT
dc.subjectColchicinept_PT
dc.subjectAmino acid sequencept_PT
dc.subjectGene expression regulationpt_PT
dc.subjectMolecular sequence datapt_PT
dc.subjectSequence alignmentpt_PT
dc.titleStructure of Tetrahymena CCTθ gene and its expression under colchicine treatmentpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage449pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage443pt_PT
oaire.citation.titleBiochimica et Biophysica Acta Gene Structure and Expressionpt_PT
oaire.citation.volume1446pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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