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In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy

2015-02Journal article Restricted access
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dc.contributor.authorLopes, Marta B.
dc.contributor.authorGonçalves, Geisa A. L.
dc.contributor.authorFelício-Silva, Daniel
dc.contributor.authorPrather, Kristala L. J.
dc.contributor.authorMonteiro, Gabriel
dc.contributor.authorPrazeres, Duarte M. F.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2016-04-20T14:20:47Z
dc.date.available2016-04-20T14:20:47Z
dc.date.issued2015-02
dc.description.abstractBACKGROUNDWhile the pharmaceutical industry keeps an eye on plasmid DNA production for new generation gene therapies, real-time monitoring techniques for plasmid bioproduction are as yet unavailable. This work shows the possibility of in situ monitoring of plasmid production in Escherichia coli cultures using a near infrared (NIR) fiber optic probe. RESULTSPartial least squares (PLS) regression models based on the NIR spectra were developed for predicting bioprocess critical variables such as the concentrations of biomass, plasmid, carbon sources (glucose and glycerol) and acetate. In order to achieve robust models able to predict the performance of plasmid production processes, independently of the composition of the cultivation medium, cultivation strategy (batch versus fed-batch) and E. coli strain used, three strategies were adopted, using: (i) E. coliDH5 cultures conducted under different media compositions and culture strategies (batch and fed-batch); (ii) engineered E. coli strains, MG1655endArecApgi and MG1655endArecA, grown on the same medium and culture strategy; (iii) diverse E. coli strains, over batch and fed-batch cultivations and using different media compositions. PLS models showed high accuracy for predicting all variables in the three groups of cultures. CONCLUSIONNIR spectroscopy combined with PLS modeling provides a fast, inexpensive and contamination-free technique to accurately monitoring plasmid bioprocesses in real time, independently of the medium composition, cultivation strategy and the E. coli strain used.pt_PT
dc.identifier.citationLOPES, Marta B. ; [et al.] - In situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategy. Journal of chemical technology and biotechnology. ISSN.0268-2575. Vol. 90, Nr. 2, SI, (2015), 255-261.pt_PT
dc.identifier.doi10.1002/jctb.4431pt_PT
dc.identifier.issn0268-2575
dc.identifier.issn1097-4660
dc.identifier.urihttp://hdl.handle.net/10400.21/6058
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley-Blackwellpt_PT
dc.relationRATIONAL ENGINEERING OF E. COLI STRAINS AND VECTORS FOR IMPROVED MANUFACTURING OF PLASMID BIOPHARMACEUTICALS
dc.relation.ispartofseriesSI
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/jctb.4431/abstractpt_PT
dc.subjectPlasmid bioprocesspt_PT
dc.subjectEscherichia colipt_PT
dc.subjectNear infrared spectroscopypt_PT
dc.subjectIn situ monitoringpt_PT
dc.titleIn situ NIR spectroscopy monitoring of plasmid production processes effect of producing strain, medium composition and the cultivation strategypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRATIONAL ENGINEERING OF E. COLI STRAINS AND VECTORS FOR IMPROVED MANUFACTURING OF PLASMID BIOPHARMACEUTICALS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F73758%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F33786%2F2009/PT
oaire.citation.endPage261pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage255pt_PT
oaire.citation.volume90pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
relation.isProjectOfPublicatione934d4a3-07d0-4c5d-8183-e76b3b0bf237
relation.isProjectOfPublication2e866b8b-53df-4729-b132-92adf509db2c
relation.isProjectOfPublication73b94df5-4a96-4e73-95fd-4f5ca1a04d71
relation.isProjectOfPublication.latestForDiscovery2e866b8b-53df-4729-b132-92adf509db2c

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