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Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives

2011-07Journal article Open access
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dc.contributor.authorPinheiro, A. C.
dc.contributor.authorMacedo, M. F.
dc.contributor.authorJurado, V.
dc.contributor.authorSaiz-Jimenez, C.
dc.contributor.authorViegas, Carla
dc.contributor.authorBrandão, J.
dc.contributor.authorRosado, L.
dc.date.accessioned2013-12-23T17:47:51Z
dc.date.available2013-12-23T17:47:51Z
dc.date.issued2011-07
dc.description.abstractThis project was developed to fully assess the indoor air quality in archives and libraries from a fungal flora point of view. It uses classical methodologies such as traditional culture media – for the viable fungi – and modern molecular biology protocols, especially relevant to assess the non-viable fraction of the biological contaminants. Denaturing high-performance liquid chromatography (DHPLC) has emerged as an alternative to denaturing gradient gel electrophoresis (DGGE) and has already been applied to the study of a few bacterial communities. We propose the application of DHPLC to the study of fungal colonization on paper-based archive materials. This technology allows for the identification of each component of a mixture of fungi based on their genetic variation. In a highly complex mixture of microbial DNA this method can be used simply to study the population dynamics, and it also allows for sample fraction collection, which can, in many cases, be immediately sequenced, circumventing the need for cloning. Some examples of the methodological application are shown. Also applied is fragment length analysis for the study of mixed Candida samples. Both of these methods can later be applied in various fields, such as clinical and sand sample analysis. So far, the environmental analyses have been extremely useful to determine potentially pathogenic/toxinogenic fungi such as Stachybotrys sp., Aspergillus niger, Aspergillus fumigatus, and Fusarium sp. This work will hopefully lead to more accurate evaluation of environmental conditions for both human health and the preservation of documents.por
dc.identifier.citationPinheiro AC, Macedo MF, Jurado V, Saiz-Jimenez C, Viegas C, Brandão J, et al. Mould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archives. Int Biodeterior Biodegradation. 2011;65(4):619-27.por
dc.identifier.issn0964-8305
dc.identifier.urihttp://hdl.handle.net/10400.21/3026
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0964830511000485por
dc.subjectFungipor
dc.subjectDHPLCpor
dc.subjectArchivespor
dc.subjectPaper documentspor
dc.subjectPortugalpor
dc.titleMould and yeast identification in archival settings: preliminary results on the use of traditional methods and molecular biology options in Portuguese archivespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage627por
oaire.citation.startPage619por
oaire.citation.titleInternational Biodeterioration & Biodegradationpor
oaire.citation.volume65por
person.familyNameViegas
person.givenNameCarla
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.orcid0000-0002-1545-6479
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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