Publication
CCTα and CCTδ chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena
| dc.contributor.author | Seixas, Cecília | |
| dc.contributor.author | Cruto, Teresa | |
| dc.contributor.author | Tavares, Alexandra | |
| dc.contributor.author | Gaertig, Jacek | |
| dc.contributor.author | Soares, Helena | |
| dc.date.accessioned | 2014-01-01T18:30:05Z | |
| dc.date.available | 2014-01-01T18:30:05Z | |
| dc.date.issued | 2010-05 | |
| dc.description.abstract | Background - The eukaryotic cytosolic chaperonin CCT is a hetero-oligomeric complex formed by two rings connected back-to-back, each composed of eight distinct subunits (CCTalpha to CCTzeta). CCT complex mediates the folding, of a wide range of newly synthesised proteins including tubulin (alpha, beta and gamma) and actin, as quantitatively major substrates. Methodology/Principal findings - We disrupted the genes encoding CCTalpha and CCTdelta subunits in the ciliate Tetrahymena. Cells lacking the zygotic expression of either CCTalpha or CCTdelta showed a loss of cell body microtubules, failed to assemble new cilia and died within 2 cell cycles. We also show that loss of CCT subunit activity leads to axoneme shortening and splaying of tips of axonemal microtubules. An epitope-tagged CCTalpha rescued the gene knockout phenotype and localized primarily to the tips of cilia. A mutation in CCTalpha, G346E, at a residue also present in the related protein implicated in the Bardet Biedel Syndrome, BBS6, also caused defects in cilia and impaired CCTalpha localization in cilia. Conclusions/Significance - Our results demonstrate that the CCT subunits are essential and required for ciliary assembly and maintenance of axoneme structure, especially at the tips of cilia. | por |
| dc.identifier.citation | Seixas C, Cruto T, Tavares A, Gaertig J, Soares H. CCTα and CCTδ chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena. PLoS One. 2010;5(5):e10704. | por |
| dc.identifier.issn | 1932-6203 | |
| dc.identifier.uri | http://hdl.handle.net/10400.21/3043 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | PLoS | por |
| dc.relation.publisherversion | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010704 | por |
| dc.subject | Amino acid substitution/genetics | por |
| dc.subject | Axoneme/metabolism | por |
| dc.subject | Axoneme/pathology | por |
| dc.subject | Chaperonin containing TCP-1/metabolism | por |
| dc.subject | Cilia/metabolism | por |
| dc.subject | Epitopes/metabolism | por |
| dc.subject | Gene Knockout techniques | por |
| dc.subject | Microtubules/metabolism | por |
| dc.subject | Mutation/genetics | por |
| dc.subject | Protein subunits/metabolism | por |
| dc.subject | Recombinant fusion proteins | por |
| dc.subject | Temperature | por |
| dc.subject | Tetrahymena | por |
| dc.subject | Zygote | por |
| dc.title | CCTα and CCTδ chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | e10704 | por |
| oaire.citation.title | PLoS ONE | por |
| oaire.citation.volume | 5 | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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