Publication
Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types
dc.contributor.author | Malta-Vacas, Joana | |
dc.contributor.author | Aires, C. | |
dc.contributor.author | Costa, P. | |
dc.contributor.author | Conde, A. R. | |
dc.contributor.author | Ramos, S. | |
dc.contributor.author | Martins, A. P. | |
dc.contributor.author | Monteiro, C. | |
dc.contributor.author | Brito, Miguel | |
dc.date.accessioned | 2013-12-10T15:52:17Z | |
dc.date.available | 2013-12-10T15:52:17Z | |
dc.date.issued | 2005-06 | |
dc.description.abstract | Background: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p , 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription. | por |
dc.identifier.citation | Malta-Vacas J, Aires C, Costa P, Conde AR, Ramos S, Martins AP, Monteiro C, Brito M. Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. J Clin Pathol. 2005 Jun;58(6):621-5. | por |
dc.identifier.issn | 1472-4146 | |
dc.identifier.uri | http://hdl.handle.net/10400.21/2982 | |
dc.language.iso | eng | por |
dc.peerreviewed | yes | por |
dc.publisher | BMJ | por |
dc.relation.publisherversion | http://jcp.bmj.com/content/58/6/621.long | por |
dc.subject | DNA, Neoplasm/genetics | por |
dc.subject | Gene dosage | por |
dc.subject | Neoplasm proteins/genetics | por |
dc.subject | Neoplasm proteins/metabolism | por |
dc.subject | Peptide termination factors/genetics | por |
dc.subject | Peptide termination factors/metabolism | por |
dc.subject | RNA, Neoplasm/genetics | por |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction/methods | por |
dc.subject | Stomach neoplasms | por |
dc.subject | Up-regulation | por |
dc.title | Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types | por |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.endPage | 625 | por |
oaire.citation.startPage | 621 | por |
oaire.citation.title | Journal of Clinical Pathology | por |
oaire.citation.volume | 58 | por |
person.familyName | Brito | |
person.givenName | Miguel | |
person.identifier.ciencia-id | 231F-F341-7E93 | |
person.identifier.orcid | 0000-0001-6394-658X | |
person.identifier.rid | A-7970-2016 | |
person.identifier.scopus-author-id | 35224551000 | |
rcaap.rights | openAccess | por |
rcaap.type | article | por |
relation.isAuthorOfPublication | 4252d8e0-800c-4d67-8b13-0b711d860669 | |
relation.isAuthorOfPublication.latestForDiscovery | 4252d8e0-800c-4d67-8b13-0b711d860669 |
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