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Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types

2005-06Journal article Open access
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dc.contributor.authorMalta-Vacas, Joana
dc.contributor.authorAires, C.
dc.contributor.authorCosta, P.
dc.contributor.authorConde, A. R.
dc.contributor.authorRamos, S.
dc.contributor.authorMartins, A. P.
dc.contributor.authorMonteiro, C.
dc.contributor.authorBrito, Miguel
dc.date.accessioned2013-12-10T15:52:17Z
dc.date.available2013-12-10T15:52:17Z
dc.date.issued2005-06
dc.description.abstractBackground: There are now several lines of evidence to suggest that protein synthesis and translation factors are involved in the regulation of cell proliferation and cancer development. Aims: To investigate gene expression patterns of eukaryotic releasing factor 3 (eRF3) in gastric cancer. Methods: RNA was prepared from 25 gastric tumour biopsies and adjacent non-neoplastic mucosa. Real time TaqMan reverse transcription polymerase chain reaction (RT-PCR) was performed to measure the relative gene expression levels. DNA was isolated from tumour and normal tissues and gene dosage was determined by a quantitative real time PCR using SYBR Green dye. Results: Different histological types of gastric tumours were analysed and nine of the 25 tumours revealed eRF3/GSPT1 overexpression; moreover, eight of the 12 intestinal type carcinomas analysed overexpressed the gene, whereas eRF3/GSPT1 was overexpressed in only one of the 10 diffuse type carcinomas (Kruskal-Wallis Test; p , 0.05). No correlation was found between ploidy and transcript expression levels of eRF3/GSPT1. Overexpression of eRF3/GSPT1 was not associated with increased translation rates because the upregulation of eRF3/GSPT1 did not correlate with increased eRF1 levels. Conclusions: Overexpression of eRF3/GSPT1 in intestinal type gastric tumours may lead to an increase in the translation efficiency of specific oncogenic transcripts. Alternatively, eRF3/GSPT1 may be involved in tumorigenesis as a result of its non-translational roles, namely (dis)regulating the cell cycle, apoptosis, or transcription.por
dc.identifier.citationMalta-Vacas J, Aires C, Costa P, Conde AR, Ramos S, Martins AP, Monteiro C, Brito M. Differential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological types. J Clin Pathol. 2005 Jun;58(6):621-5.por
dc.identifier.issn1472-4146
dc.identifier.urihttp://hdl.handle.net/10400.21/2982
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBMJpor
dc.relation.publisherversionhttp://jcp.bmj.com/content/58/6/621.longpor
dc.subjectDNA, Neoplasm/geneticspor
dc.subjectGene dosagepor
dc.subjectNeoplasm proteins/geneticspor
dc.subjectNeoplasm proteins/metabolismpor
dc.subjectPeptide termination factors/geneticspor
dc.subjectPeptide termination factors/metabolismpor
dc.subjectRNA, Neoplasm/geneticspor
dc.subjectReverse Transcriptase Polymerase Chain Reaction/methodspor
dc.subjectStomach neoplasmspor
dc.subjectUp-regulationpor
dc.titleDifferential expression of the eukaryotic release factor 3 (eRF3/GSPT1) according to gastric cancer histological typespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage625por
oaire.citation.startPage621por
oaire.citation.titleJournal of Clinical Pathologypor
oaire.citation.volume58por
person.familyNameBrito
person.givenNameMiguel
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublication.latestForDiscovery4252d8e0-800c-4d67-8b13-0b711d860669

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