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Authors
Abstract(s)
O uso intensivo de pesticidas, como atrazina (ATZ) e glifosato (GLY) aumentou exponencialmente, estando associados a efeitos nocivos na saúde humana e animal. Este estudo avaliou os efeitos de ATZ e GLY na viabilidade, motilidade (cauda) e potencial de membrana mitocondrial (ΔΨm) de espermatozoides epididimários bovinos. Amostras isoladas de espermatozoides epididimários (cabeça, corpo e cauda) de 11 touros Holstein-Frísia foram expostos a diferentes concentrações de ATZ (0,1, 1 e 10 μM) e GLY (5, 50 e 360 mg/L), e os parâmetros foram avaliados antes e após 2 e 4 horas de exposição. Os resultados indicaram que a viabilidade dos espermatozoides não foi afetada pela ATZ até 4 horas, embora tenha havido uma redução na proporção de espermatozoides vivos entre 2 e 4 horas para espermatozoides da cabeça e corpo, mas não da cauda. A motilidade subjetiva não foi afetada pela ATZ até 4 horas, embora um declínio foi observado entre 2 e 4 horas. Para o ΔΨm, observou-se diferenças entre as 2 e as 4 horas de incubação de amostras obtidas da cauda do epidídimo com 1 μM de ATZ. O GLY não mostrou efeitos na motilidade e apenas espermatozoides da cabeça mostraram uma diminuição na viabilidade entre 2 e 4 horas. O ΔΨm não foi alterado pelas concentrações do GLY em nenhum dos tempos de incubação. Os resultados sugerem que marcadores convencionais podem não detetar efeitos subtis da ATZ e GLY nos espermatozoides, mas análises de metabolómica e de stress oxidativo podem fornecer uma avaliação mais abrangente e o desenvolvimento de biomarcadores sensíveis.
ABSTRACT - The intensive use of pesticides such as atrazine (ATZ) and glyphosate (GLY) has increased exponentially, and they are associated with harmful effects on human and animal health. This study evaluated the effects of ATZ and GLY on the viability, motility (tail) and mitochondrial membrane potential (ΔΨm) of bovine epididymal sperm. Isolated samples of epididymal sperm (head, body and tail) from 11 Holstein-Friesian bulls were exposed to different concentrations of ATZ (0.1, 1 and 10 μM) and GLY (5, 50 and 360 mg/L), and the parameters were assessed before and after 2 and 4 hours of exposure. The results indicated that sperm viability was not affected by ATZ up to 4 hours, although there was a reduction in the proportion of live sperm between 2 and 4 hours for sperm from the head and body, but not from the tail. Subjective motility was not affected by ATZ up to 4 hours, although a decline was observed between 2 and 4 hours. To ΔΨm, a difference was observed between the 2 and 4 hours of incubation of samples obtained from the cauda epididymis with 1 μM ATZ. GLY showed no effect on motility and only sperm from the head showed a decrease in viability between 2 and 4 hours. ΔΨm was not altered by GLY concentrations at any of the incubation times. The results suggest that conventional markers may not detect subtle effects of ATZ and GLY on sperm, but metabolomics and oxidative stress analyses may provide a more comprehensive assessment and the development of sensitive biomarkers.
ABSTRACT - The intensive use of pesticides such as atrazine (ATZ) and glyphosate (GLY) has increased exponentially, and they are associated with harmful effects on human and animal health. This study evaluated the effects of ATZ and GLY on the viability, motility (tail) and mitochondrial membrane potential (ΔΨm) of bovine epididymal sperm. Isolated samples of epididymal sperm (head, body and tail) from 11 Holstein-Friesian bulls were exposed to different concentrations of ATZ (0.1, 1 and 10 μM) and GLY (5, 50 and 360 mg/L), and the parameters were assessed before and after 2 and 4 hours of exposure. The results indicated that sperm viability was not affected by ATZ up to 4 hours, although there was a reduction in the proportion of live sperm between 2 and 4 hours for sperm from the head and body, but not from the tail. Subjective motility was not affected by ATZ up to 4 hours, although a decline was observed between 2 and 4 hours. To ΔΨm, a difference was observed between the 2 and 4 hours of incubation of samples obtained from the cauda epididymis with 1 μM ATZ. GLY showed no effect on motility and only sperm from the head showed a decrease in viability between 2 and 4 hours. ΔΨm was not altered by GLY concentrations at any of the incubation times. The results suggest that conventional markers may not detect subtle effects of ATZ and GLY on sperm, but metabolomics and oxidative stress analyses may provide a more comprehensive assessment and the development of sensitive biomarkers.
Description
Mestrado em Tecnologias Clínico-Laboratoriais
Keywords
Toxicidade Reprodução Herbicidas Espermatozoides bovinos Toxicity Reproduction Herbicides Bovine spermatozoa MTCL
Citation
Pereira AA. Efeito da atrazina e do glifosato na função e viabilidade de espermatozoides epididimários [dissertation]. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa/Instituto Politécnico de Lisboa; 2024.
Publisher
Instituto Politécnico de Lisboa, Escola Superior de Tecnologia da Saúde de Lisboa