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Impact of neutrophil-activating protein conservation on diagnostic tests and vaccine design

2024-09Journal article Open access
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dc.contributor.authorGonçalves, Lídia M. D.
dc.contributor.authorAlmeida, António J.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2024-11-04T08:36:59Z
dc.date.available2024-11-04T08:36:59Z
dc.date.issued2024-09
dc.description.abstractBACKGROUND: The neutrophil activating protein (NAP) is a highly immunogenic and virulence factor of Helicobacter pylori, pre-senting inflammatory and immunomodulatory activity. Consequently, NAP has been explored as a diagnostic and therapeutictarget. However, when evaluating a target protein to design diagnostic methods or vaccines, it is critical to determine the pro-tein conservation among the bacterial population, as well the impact of alterations of amino acid residues on the protein anti-genic profile. RESULTS: In the present work, NAP conservation and theoretical antigenicity were determined among 51 sequences fromH. pylori isolated from patients worldwide. A high NAP conservation (83%) was observed, where 17 amino acid residues, amongthe 144 residues of the protein, were polymorphic. Alterations at these polymorphic sites had a theoretically low impact on pre-dicted antigenicity, where only 5 NAPs out of 51 NAPs presented a slightly different antigenic profile in relation to the consen-sus sequence. According to that, it was possible to recognize in western blotting 93% of NAP from different bacteria (n = 15) using polyclonal antibodies developed against a specific NAP. CONCLUSIONS: It was predicted that when working with polyclonal antibodies or large NAP fragments for diagnostic and vaccine design, slight variation in protein sequence will have a minimal impact on NAP recognition. However, if a NAP monoclonal antibody or small NAP epitopes are considered, it is critical to select the most conserved and antigenic NAP regions, to maximize the coverage of NAP variants.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGONÇALVES, Lídia M. D.; ALMEIDA, António J.; CALADO, Cecília R. C. – Impact of neutrophil-activating protein conservation on diagnostic tests and vaccine design. Journal of Chemical Technology and Biotechnology. eISSN 1097-4660. (2024), https://doi.org/10.1002/jctb.7755pt_PT
dc.identifier.doi10.1002/jctb.7755pt_PT
dc.identifier.eissn1097-4660
dc.identifier.urihttp://hdl.handle.net/10400.21/17824
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationResearch Institute for Medicines
dc.subjectHelicobacter pyloript_PT
dc.subjectNAPpt_PT
dc.subjectimmunogenicpt_PT
dc.subjectgenetic variabilitypt_PT
dc.subjectneutrophil-activating proteinpt_PT
dc.subjectimmunovariabilitypt_PT
dc.titleImpact of neutrophil-activating protein conservation on diagnostic tests and vaccine designpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Institute for Medicines
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2013/PT
oaire.citation.endPage10pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Chemical Technology and Biotechnologypt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCalado
person.givenNameCecília
person.identifier130332
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id6603163260
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc
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