Publicação
Impact of neutrophil-activating protein conservation on diagnostic tests and vaccine design
| dc.contributor.author | Gonçalves, Lídia M. D. | |
| dc.contributor.author | Almeida, António J. | |
| dc.contributor.author | Calado, Cecília | |
| dc.date.accessioned | 2024-11-04T08:36:59Z | |
| dc.date.available | 2024-11-04T08:36:59Z | |
| dc.date.issued | 2024-09 | |
| dc.description.abstract | BACKGROUND: The neutrophil activating protein (NAP) is a highly immunogenic and virulence factor of Helicobacter pylori, pre-senting inflammatory and immunomodulatory activity. Consequently, NAP has been explored as a diagnostic and therapeutictarget. However, when evaluating a target protein to design diagnostic methods or vaccines, it is critical to determine the pro-tein conservation among the bacterial population, as well the impact of alterations of amino acid residues on the protein anti-genic profile. RESULTS: In the present work, NAP conservation and theoretical antigenicity were determined among 51 sequences fromH. pylori isolated from patients worldwide. A high NAP conservation (83%) was observed, where 17 amino acid residues, amongthe 144 residues of the protein, were polymorphic. Alterations at these polymorphic sites had a theoretically low impact on pre-dicted antigenicity, where only 5 NAPs out of 51 NAPs presented a slightly different antigenic profile in relation to the consen-sus sequence. According to that, it was possible to recognize in western blotting 93% of NAP from different bacteria (n = 15) using polyclonal antibodies developed against a specific NAP. CONCLUSIONS: It was predicted that when working with polyclonal antibodies or large NAP fragments for diagnostic and vaccine design, slight variation in protein sequence will have a minimal impact on NAP recognition. However, if a NAP monoclonal antibody or small NAP epitopes are considered, it is critical to select the most conserved and antigenic NAP regions, to maximize the coverage of NAP variants. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | GONÇALVES, Lídia M. D.; ALMEIDA, António J.; CALADO, Cecília R. C. – Impact of neutrophil-activating protein conservation on diagnostic tests and vaccine design. Journal of Chemical Technology and Biotechnology. eISSN 1097-4660. (2024), https://doi.org/10.1002/jctb.7755 | pt_PT |
| dc.identifier.doi | 10.1002/jctb.7755 | pt_PT |
| dc.identifier.eissn | 1097-4660 | |
| dc.identifier.uri | http://hdl.handle.net/10400.21/17824 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Wiley | pt_PT |
| dc.relation | Research Institute for Medicines | |
| dc.subject | Helicobacter pylori | pt_PT |
| dc.subject | NAP | pt_PT |
| dc.subject | immunogenic | pt_PT |
| dc.subject | genetic variability | pt_PT |
| dc.subject | neutrophil-activating protein | pt_PT |
| dc.subject | immunovariability | pt_PT |
| dc.title | Impact of neutrophil-activating protein conservation on diagnostic tests and vaccine design | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardNumber | DSAIPA/DS/0117/2020 | |
| oaire.awardNumber | PTDC/BIO/69242/2006 | |
| oaire.awardNumber | UID/DTP/04138/2013 | |
| oaire.awardTitle | Research Institute for Medicines | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/DSAIPA%2FDS%2F0117%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBIO%2F69242%2F2006/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FDTP%2F04138%2F2013/PT | |
| oaire.citation.endPage | 10 | pt_PT |
| oaire.citation.startPage | 1 | pt_PT |
| oaire.citation.title | Journal of Chemical Technology and Biotechnology | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| person.familyName | Calado | |
| person.givenName | Cecília | |
| person.identifier | 130332 | |
| person.identifier.ciencia-id | 9418-E320-3177 | |
| person.identifier.orcid | 0000-0002-5264-9755 | |
| person.identifier.rid | E-2102-2014 | |
| person.identifier.scopus-author-id | 6603163260 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
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