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Aspergillus spp. prevalence in different Portuguese occupational environments: what is the real scenario in high load settings?

2017-10Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authorCaetano, Liliana Aranha
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Quintal
dc.contributor.authorViegas, Susana
dc.date.accessioned2018-01-17T10:44:02Z
dc.date.available2018-01-17T10:44:02Z
dc.date.issued2017-10
dc.description.abstractThe genus Aspergillus is one of the most prevalent regarding fungi in several highly contaminated occupational environments. The goal of the current study was to assess the prevalence of Aspergillus spp. in different settings, focusing on those where a higher load of fungal contamination is expected according to the European Agency for Safety and Health at Work. A specific protocol to ensure a more accurate assessment of the exposure to Aspergillus spp. is proposed aimed at allowing a detailed risk characterization and management. Two wastewater treatment plants, one wastewater elevation plant, four waste treatment plants, three cork industries, five slaughterhouses, four feed industries, one poultry pavilion, and two swineries, all located in the outskirts of Lisbon, were assessed. In total, 125 air samples and 125 surface samples were collected and analyzed by culture-based methods. Real-time polymerase chain reaction was performed to detect the fungal presence in 100 samples, targeting the Aspergillus sections Circumdati, Flavi, and Fumigati. The highest prevalence of Aspergillus spp. was found in wastewater treatment plants (69.3%; 31.1%), waste treatment plants (34.8%; 73.6%), and poultry feed industry (6.3%; 26.1%), in air and surfaces, respectively. Aspergillus spp. was also prevalent in cork industry (0.9%; 23.4%), slaughterhouses (1.6%; 17.7%), and swineries (7.4%; 9.5%), in air and surfaces, respectively. The Aspergillus sections more prevalent in the air and surfaces of all the assessed settings were the Nigri section (47.46%; 44.71%, respectively), followed by Fumigati (22.28%; 27.97%, respectively) and Flavi (10.78%; 11.45%, respectively) sections. Aspergillus section Fumigati was successfully amplified by qPCR in 18 sampling sites where the presence of this fungal species had not been identified by conventional methods. It should be highlighted that the occupational exposure burden is due not only to the Aspergillus load but also to the toxigenic potential of this genus. Based on our results, a protocol relied on the application of conventional and molecular methods in parallel is herein suggested aimed at allowing a better risk characterization and management.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Faria T, Caetano LA, Carolino E, Gomes AQ, Viegas S. Aspergillus spp. prevalence in different Portuguese occupational environments: what is the real scenario in high load settings? J Occup Environ Hyg. 2017;14(10):771-85.pt_PT
dc.identifier.doi10.1080/15459624.2017.1334901pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7927
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttp://www.tandfonline.com/doi/full/10.1080/15459624.2017.1334901pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectAspergilluspt_PT
dc.subjectCircumdatipt_PT
dc.subjectFlavipt_PT
dc.subjectAspergillus fumigatuspt_PT
dc.subjectRisk characterizationpt_PT
dc.titleAspergillus spp. prevalence in different Portuguese occupational environments: what is the real scenario in high load settings?pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage785pt_PT
oaire.citation.issue10pt_PT
oaire.citation.startPage771pt_PT
oaire.citation.titleJournal of Occupational and Environmental Hygienept_PT
oaire.citation.volume14pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery13115332-43f7-4048-a8a5-2f2b855a8c92

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