Publication
MicroRNA-146a controls functional plasticity in γδ T cells by targeting NOD1
dc.contributor.author | Schmolka, Nina | |
dc.contributor.author | Papotto, Pedro H. | |
dc.contributor.author | Romero, Paula Vargas | |
dc.contributor.author | Amado, Tiago | |
dc.contributor.author | Enguita, Francisco J. | |
dc.contributor.author | Amorim, Ana | |
dc.contributor.author | Rodrigues, Ana F. | |
dc.contributor.author | Gordon, Katrina E. | |
dc.contributor.author | Coroadinha, Ana S. | |
dc.contributor.author | Boldin, Mark | |
dc.contributor.author | Serre, Karine | |
dc.contributor.author | Buck, Amy H. | |
dc.contributor.author | Gomes, Anita Quintal | |
dc.contributor.author | Silva-Santos, Bruno | |
dc.date.accessioned | 2018-06-11T16:03:10Z | |
dc.date.available | 2018-06-11T16:03:10Z | |
dc.date.issued | 2018-05 | |
dc.description.abstract | γδ T cells are major providers of proinflammatory cytokines. They are preprogrammed in the mouse thymus into distinct subsets producing either interleukin-17 (IL-17) or interferon-γ (IFN-γ), which segregate with CD27 expression. In the periphery, CD27- γδ (γδ27-) T cells can be induced under inflammatory conditions to coexpress IL-17 and IFN-γ; the molecular basis of this functional plasticity remains to be determined. On the basis of differential microRNA (miRNA) expression analysis and modulation in γδ T cell subsets, we identified miR-146a as a thymically imprinted post-transcriptional brake to limit IFN-γ expression in γδ27- T cells in vitro and in vivo. On the basis of biochemical purification of Argonaute 2-bound miR-146a targets, we identified Nod1 to be a relevant mRNA target that regulates γδ T cell plasticity. In line with this, Nod1-deficient mice lacked multifunctional IL-17+ IFN-γ+ γδ27- cells and were more susceptible to Listeria monocytogenes infection. Our studies establish the miR-146a/NOD1 axis as a key determinant of γδ T cell effector functions and plasticity. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | Schmolka N, Papotto PH, Romero PV, Amado T, Enguita FJ, Gomes AQ, et al. MicroRNA-146a controls functional plasticity in γδ T cells by targeting NOD1. Sci Immunol. 2018;3(23). | pt_PT |
dc.identifier.doi | 10.1126/sciimmunol.aao1392 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.21/8618 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | Science | pt_PT |
dc.relation.publisherversion | http://immunology.sciencemag.org/content/3/23/eaao1392.long | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | T cells | pt_PT |
dc.subject | Proinflammatory cytokines | pt_PT |
dc.subject | PTDC/BEX-BCM/3592/2014 | pt_PT |
dc.title | MicroRNA-146a controls functional plasticity in γδ T cells by targeting NOD1 | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BCM%2F3592%2F2014/PT | |
oaire.citation.issue | 23 | pt_PT |
oaire.citation.startPage | eaao1392 | pt_PT |
oaire.citation.title | Science Immunology | pt_PT |
oaire.citation.volume | 3 | pt_PT |
oaire.fundingStream | 3599-PPCDT | |
project.funder.identifier | http://doi.org/10.13039/501100001871 | |
project.funder.name | Fundação para a Ciência e a Tecnologia | |
rcaap.rights | restrictedAccess | pt_PT |
rcaap.type | article | pt_PT |
relation.isProjectOfPublication | 70eddb2f-fa08-408b-8ac9-a2db43a95b39 | |
relation.isProjectOfPublication.latestForDiscovery | 70eddb2f-fa08-408b-8ac9-a2db43a95b39 |
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