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Supercritical CO2 extraction of secondary metabolites from Agaricus blazei: experiments and modelling

dc.contributor.authorCoelho, José P.
dc.contributor.authorCasquinha, Lúcia
dc.contributor.authorVelez, António
dc.contributor.authorKarmali, Amin
dc.date.accessioned2011-11-22T21:40:22Z
dc.date.available2011-11-22T21:40:22Z
dc.date.issued2009-05
dc.description.abstractThe mycelium and young fruiting bodies of Agaricus blazei were submitted to supercritical CO2 extraction, in a modified commercial flow apparatus, at temperatures from 40 to 80 ºC, pressures up to 600 bar and CO2 flow-rates from 2.0 to 9.0 g.min-1. The best extraction conditions of secondary metabolites, whereby the degree of solubilization (g extract/100 g of fungi) is the highest, was obtained with pure CO2 at 400 bar, 70 ºC and a CO2 flow rate of 5.7g.min-1. The extract in that conditions were analysed by GC-Ms. In order to increase the extraction yield of secondary metabolites, which are mostly present in glycolipid fractions, a polar compound (ethanol) was used as co-solvent in the proportions of 5 and 10 % (mol/mol). The presence of ethanol increased the yield when compared with the extraction with pure CO2. Moreover, a simple model was applied to the supercritical CO2 extraction of secondary metabolites from Agaricus blazei.por
dc.description.sponsorshipProjects: IPL 19/2003, IPL 20 /2003 and IPL 25/2003. Lúcia Casquinha was also support with a grant from Project IPL 20/2003.por
dc.identifier.citationCoelho JP, Casquinha L, Velez A, Karmali A. Supercritical CO2 extraction of secondary metabolites from Agaricus blazei: experiments and modelling. Alicerces. 2009;II(2):7-15.por
dc.identifier.issn1645-7943
dc.identifier.urihttp://hdl.handle.net/10400.21/475
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherInstituto Politécnico de Lisboapor
dc.titleSupercritical CO2 extraction of secondary metabolites from Agaricus blazei: experiments and modellingpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage15por
oaire.citation.startPage7por
oaire.citation.titleAlicercespor
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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