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The comet assay in animal models: from bugs to whales (Part 1: invertebrates)

2019-03Journal article Restricted access
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dc.contributor.authorGajski, Goran
dc.contributor.authorŽegura, Bojana
dc.contributor.authorLadeira, Carina
dc.contributor.authorPourrut, Bertrand
dc.contributor.authorDel Bo’, Cristian
dc.contributor.authorNovak, Matjaž
dc.contributor.authorSramkova, Monika
dc.contributor.authorMilić, Mirta
dc.contributor.authorGutzkow, Kristine Bjerve
dc.contributor.authorCosta, Solange
dc.contributor.authorDusinska, Maria
dc.contributor.authorBrunborg, Gunnar
dc.contributor.authorCollins, Andrew
dc.date.accessioned2019-03-22T12:41:49Z
dc.date.available2019-03-22T12:41:49Z
dc.date.issued2019-03
dc.descriptionCA COST Action CA15132 – The comet assay as a human biomonitoring tool (hCOMET)pt_PT
dc.description.abstractThe comet assay, also called single cell gel electrophoresis, is a sensitive, rapid and low-cost technique for quantifying and analyzing DNA damage and repair at the level of individual cells. The assay itself can be applied on virtually any cell type derived from different organs and tissues of eukaryotic organisms. Although it is mainly used on human cells, the assay has applications also in the evaluation of DNA damage in yeast, plant and animal cells. Therefore, the purpose of this review is to give an extensive overview of the usage of the comet assay in animal models from invertebrates to vertebrates, covering both terrestrial and water biota. The comet assay is used in a variety of invertebrate species since they are regarded as interesting subjects in ecotoxicological research due to their significance in ecosystems. Hence, the first part of the review (Part 1) will discuss the application of the comet assay in invertebrates covering protozoans, platyhelminthes, planarians, cnidarians, mollusks, annelids, arthropods, and echinoderms. Besides a large number of animal species, the assay is also performed on a variety of cells, which includes haemolymph, gills, digestive gland, sperm and embryo cells. The mentioned cells have been used for the evaluation of a broad spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of invertebrate models and their role from an ecotoxicological point of view will also be discussed as well as the comparison of the use of the comet assay in invertebrate and human models. Since the comet assay is still developing, its increasing potential in assessing DNA damage in animal models is crucial especially in the field of ecotoxicology and biomonitoring at the level of different species, not only humans.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGajski G, Žegura B, Ladeira C, Pourrut B, Del Bo’ C, Novak M, et al. The comet assay in animal models: from bugs to whales (Part 1: invertebrates). Mutat Res Rev Mutat Res. 2019;779:82-113.pt_PT
dc.identifier.doi10.1016/j.mrrev.2019.02.003pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/9751
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCA COST Action CA15132pt_PT
dc.relationhCOMETpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383574218300589pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectComet assaypt_PT
dc.subjectDNA damagept_PT
dc.subjectInvertebratespt_PT
dc.subjectIn vitropt_PT
dc.subjectIn vivopt_PT
dc.subjectBiomonitoringpt_PT
dc.titleThe comet assay in animal models: from bugs to whales (Part 1: invertebrates)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage113pt_PT
oaire.citation.startPage82pt_PT
oaire.citation.titleMutation Research - Reviewspt_PT
oaire.citation.volume779pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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