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The contribution of cellblock – histogel – in bronchial washing samples

2014-05Conference object Open access
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dc.contributor.authorPatraquim, Catarina
dc.contributor.authorMartins, Iolanda
dc.contributor.authorMarques, Ricardo
dc.contributor.authorMendonça, Paula
dc.contributor.authorLeitão, Dina
dc.date.accessioned2016-01-14T16:47:38Z
dc.date.available2016-01-14T16:47:38Z
dc.date.issued2014-05
dc.description.abstractIntroduction: The cellblock is a technique that enables the pathologist to study the morphological detail of residual samples and can be used when it is necessary to perform additional diagnostic techniques. Objective: Demonstrate the processing of bronchial washings in liquid based cytology to cellblock using HistoGel in residual samples, evaluating the morphology and preservation of cytological material. Methods: There were used 40 residual samples from bronchial washings in liquid based cytology, after determination of the clinical diagnosis, being made subsequently 40 cellblocks using HistoGel. For each cellblock there was made one histological section for analysis of cell morphology, which was subsequently stained with the routine histological staining. After microscope observation, the morphology was evaluated by 3 experts in the field of pathology, based on the parameters: Cellularity, Preservation and Background. Results: The average final score of 3 evaluators, on a scale of 0 to 100, in assessing the morphology of the 40 samples was 55.6. From the 40 histological sections, 5 of them were considered not viable for evaluation. Conclusions: The results obtained indicate median quality maintenance of morphology. However, it is noted that in only 5 cases it was not possible to determine an evaluation, knowing from the outset that these are residual samples with a very scant cellularity. Thus, it is possible to say that the processing of bronchial washings to cellblock using HistoGel contributes to a concentration of the cytological material, allowing its evaluation and subsequent diagnosis. Additional diagnostic techniques are shown equally viable in these cellblocks.pt_PT
dc.identifier.citationPatraquim C, Martins I, Marques R, Mendonça P, Leitão D. The contribution of cellblock – histogel – in bronchial washing samples. In 2nd IPLeiria International Health Congress – Challenges & Innovation in Health, Leiria (Portugal), 9th to 10th May 2014. Rev Saude Publica. 2014;48(sp. no.):285.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/5551
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://www.health.ipleiria.pt/files/2014/06/RSPv048n.esp-congresso-book.pdfpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectBronchial washingspt_PT
dc.subjectCellblockpt_PT
dc.subjectCell morphologypt_PT
dc.subjectCytologypt_PT
dc.subjectHistoGelpt_PT
dc.titleThe contribution of cellblock – histogel – in bronchial washing samplespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.endPage285pt_PT
oaire.citation.startPage285pt_PT
oaire.citation.titleRevista de Saúde Públicapt_PT
oaire.citation.volume48pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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