Repository logo
 
Publication

Optimisation and economic assessment of lipase-catalysed production of monoesters using Rhizomucor miehei lipase in a solvent-free system

2016-11Journal article Restricted access
Simple item page
dc.contributor.authorAhmad, Mustafa
dc.contributor.authorKarmali, Amin
dc.contributor.authorAbdelmoez, Wael
dc.date.accessioned2017-06-06T12:15:37Z
dc.date.available2017-06-06T12:15:37Z
dc.date.issued2016-11
dc.description.abstractEnzymatic production of glycerin monolaurate (GML) was carried out by lipase catalysed esterification of lauric acid and glycerin in a solvent-free media. Response surface methodology (RSM), based on 5-level and 3-variable composite design, was employed to study the interactive effects of reaction temperature (48-60 degrees C), enzyme load (1-4% w/w), and glycerin to lauric acid molar ratio (1:1-4:1) on glycerin laurate yield. The optimum conditions obtained were a temperature of 60 degrees C, an enzyme load of 4%, and a glycerin to lauric acid molar ratio of 4:1. The maximum predicted and experimental conversion values were 92.26% and 93.23%, respectively. Utilisation of Lipozyme RM IM (Rhizomucor miehei lipase) allowed the formation of a mixture consisting of 50% monoglyceride, 34.6% diglyceride and 8A% triglyceride, which fulfills the requirements established by the World Health Organization (WHO) for use as a food emulsifier. In addition, the Lipozyme RM IM maintained more than 90% of its original activity after being used for six cycles. Finally, an economic study was performed to interrogate the feasibility of the proposed enzymatic manufacturing process. The obtained results were Compared with the traditional catalysed chemical process. The economic feasibility study revealed that the needed amount of Lipozyme RM IM was 2.5 kg/t of feedstock. The high conversion achieved in a short reaction time (1 h), as well as the proven operational stability of Lipozyme RM IM, revealed a promising potential for this green and sustainable route in practical applications.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMUSTAFA, Ahmad; KARMALI, Amin; ABDELMOEZ, Wael - Optimisation and economic assessment of lipase-catalysed production of monoesters using Rhizomucor miehei lipase in a solvent-free system. ISSN: 0959-6526. Vol. 137, (2016), pp. 953-964pt_PT
dc.identifier.doi10.1016/j.jclepro.2016.07.056pt_PT
dc.identifier.issn0959-6526
dc.identifier.urihttp://hdl.handle.net/10400.21/7155
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://ac.els-cdn.com/S0959652616309441/1-s2.0-S0959652616309441-main.pdf?_tid=f2b56cac-4524-11e7-bf8e-00000aacb35d&acdnat=1496141245_c38de1313fa0b2a05499482df11ace8ept_PT
dc.subjectGlycerin lauratept_PT
dc.subjectLipozyme RM IMpt_PT
dc.subjectResponse surface methodologypt_PT
dc.subjectEsterificationpt_PT
dc.subjectEconomic assessmentpt_PT
dc.titleOptimisation and economic assessment of lipase-catalysed production of monoesters using Rhizomucor miehei lipase in a solvent-free systempt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage964pt_PT
oaire.citation.startPage953pt_PT
oaire.citation.titleJournal of Cleaner Productionpt_PT
oaire.citation.volume137pt_PT
person.familyNameKarmali
person.familyNameAbdelmoez
person.givenNameAmin
person.givenNameWael
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0002-5370-7646
person.identifier.ridE-4787-2011
person.identifier.scopus-author-id6603778216
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublication857e4d18-b0e5-45d6-8e10-e30adf61e3d3
relation.isAuthorOfPublication.latestForDiscovery857e4d18-b0e5-45d6-8e10-e30adf61e3d3

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Optimisation and economic_AKarnali_ADQ.pdf
Size:
2.33 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEL - Eng. Quim. Biol. - Artigos