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Multi-mycotoxin analysis using dried blood spots and dried serum spots

2017-03Journal article Restricted access
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dc.contributor.authorOsteresch, Bernd
dc.contributor.authorViegas, Susana
dc.contributor.authorCramer, Benedikt
dc.contributor.authorHumpf, Hans-Ulrich
dc.date.accessioned2017-05-16T15:11:11Z
dc.date.available2017-05-16T15:11:11Z
dc.date.issued2017-03
dc.description.abstractIn this study, a rapid multi-mycotoxin approach was developed for biomonitoring and quantification of 27 important mycotoxins and mycotoxin metabolites in human blood samples. HPLC-MS/MS detection was used for the analysis of dried serum spots (DSS) and dried blood spots (DBS). Detection of aflatoxins (AFB1, AFB2, AFG1, AFG2, AFM1), trichothecenes (deoxynivalenol, DON; DON-3-glucoronic acid, DON-3-GlcA; T-2; HT-2; and HT-2-4-GlcA), fumonisin B1 (FB1), ochratoxins (OTA and its thermal degradation product 2'R-OTA; OTα; 10-hydroxychratoxin A, 10-OH-OTA), citrinin (CIT and its urinary metabolite dihydrocitrinone, DH-CIT), zearalenone and zearalanone (ZEN, ZAN), altenuene (ALT), alternariols (AOH; alternariol monomethyl ether, AME), enniatins (EnA, EnA1, EnB, EnB1) and beauvericin (Bea) was validated for two matrices, serum (DSS), and whole blood (DBS). HPLC-MS/MS analysis showed signal suppression as well as signal enhancement due to matrix effects. However, for most analytes LOQs in the lower pg/mL range and excellent recovery rate were achieved using matrix-matched calibration. Besides validation of the method, the analyte stability in DBS and DSS was also investigated. Stability is a main issue for some analytes when the dried samples are stored under common conditions at room temperature. Nevertheless, the developed method was applied to DBS samples of a German cohort (n = 50). Besides positive findings of OTA and 2'R-OTA, all samples were positive for EnB. This methodical study establishes a validated multi-mycotoxin approach for the detection of 27 mycotoxins and metabolites in dried blood/serum spots based on a fast sample preparation followed by sensitive HPLC-MS/MS analysis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOsteresch B, Viegas S, Cramer B, Humpf HU. Multi-mycotoxin analysis using dried blood spots and dried serum spots. Anal Bioanal Chem. 2017;409(13):3369-82.pt_PT
dc.identifier.doi10.1007/s00216-017-0279-9pt_PT
dc.identifier.issn1618-2642
dc.identifier.urihttp://hdl.handle.net/10400.21/7015
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs00216-017-0279-9pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/pt_PT
dc.subjectBiomonitoringpt_PT
dc.subjectDried blood spotpt_PT
dc.subjectDried serum spotpt_PT
dc.subjectHPC-MS/MSpt_PT
dc.subjectMass spectrometrypt_PT
dc.subjectMycotoxinpt_PT
dc.titleMulti-mycotoxin analysis using dried blood spots and dried serum spotspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3382pt_PT
oaire.citation.startPage3369pt_PT
oaire.citation.titleAnalytical and Bioanalytical Chemistrypt_PT
oaire.citation.volume409pt_PT
person.familyNameViegas
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id35270591500
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery13115332-43f7-4048-a8a5-2f2b855a8c92

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