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DNA repair as a human biomonitoring tool: comet assay approaches

2019-03Journal article Restricted access
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dc.contributor.authorAzqueta, Amaya
dc.contributor.authorLangie, Sabine A. S.
dc.contributor.authorBoutet-Robinet, Elisa
dc.contributor.authorDuthie, Susan
dc.contributor.authorLadeira, Carina
dc.contributor.authorMøller, Peter
dc.contributor.authorCollins, Andrew
dc.contributor.authorGodschalk, Roger W. L.
dc.date.accessioned2019-03-22T17:19:54Z
dc.date.available2019-03-22T17:19:54Z
dc.date.issued2019-03
dc.descriptionhCOMET project (COST Action, CA 15132)pt_PT
dc.description.abstractThe comet assay offers the opportunity to measure both DNA damage and repair. Various comet assay based methods are available to measure DNA repair activity, but some requirements should be met for their effective use in human biomonitoring studies. These conditions include i) robustness of the assay, ii) sources of inter- and intra-individual variability must be known, iii) DNA repair kinetics should be assessed to optimize sampling timing, and iv) DNA repair in accessible surrogate tissues should reflect repair activity in target tissues prone to carcinogenic effects. DNA repair phenotyping can be performed on frozen and fresh samples and is a more direct measurement than genomic or transcriptomic approaches. There are mixed reports concerning the regulation of DNA repair by environmental and dietary factors. In general, exposure to genotoxic agents did not change base excision repair (BER) activity, whereas some studies reported that dietary interventions affected BER activity. On the other hand, in vitro, and in vivo studies indicated that nucleotide excision repair (NER) can be altered by exposure to genotoxic agents, but studies on other lifestyle-related factors, such as diet, are rare. Thus, crucial questions concerning the factors regulating DNA repair and inter-individual variation remain unanswered. Intra-individual variation over a period of days to weeks seems limited, which is favourable for DNA repair phenotyping in biomonitoring studies. Despite this reported low intra-individual variation, the timing of sampling remains an issue that needs further investigation. A correlation was reported between the repair activity in easily accessible peripheral blood mononuclear cells (PBMCs) and internal organs for both NER and BER. However, no correlation was found between tumour tissue and blood cells. In conclusion, although comet assay based approaches to measure BER/NER phenotypes are feasible and promising; more work is needed to further optimize their application in human biomonitoring and intervention studies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAzqueta A, Langie SA, Boutet-Robinet E, Duthie S, Ladeira C, Møller P, et al. DNA repair as a human biomonitoring tool: comet assay approaches. Mutat Res Rev Mutat Res. 2019;781:71-87.pt_PT
dc.identifier.doi10.1016/j.mrrev.2019.03.002pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/9761
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationhCOMET project (COST Action, CA 15132)pt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383574218300899pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDNA repairpt_PT
dc.subjectComet assaypt_PT
dc.subjectHuman biomonitoringpt_PT
dc.subjectValidationpt_PT
dc.titleDNA repair as a human biomonitoring tool: comet assay approachespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage87pt_PT
oaire.citation.startPage71pt_PT
oaire.citation.titleMutation Research - Reviewspt_PT
oaire.citation.volume781pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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