Repository logo
 
Publication

Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies

2021-05Journal article Open access
Simple item page
dc.contributor.authorMøller, Peter
dc.contributor.authorBankoglu, Ezgi Eyluel
dc.contributor.authorStopper, Helga
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorLadeira, Carina
dc.contributor.authorKoppen, Gudrun
dc.contributor.authorGajski, Goran
dc.contributor.authorCollins, Andrew
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorLaffon, Blanca
dc.contributor.authorBoutet-Robinet, Elisa
dc.contributor.authorPerdry, Hervé
dc.contributor.authorDel Bo’, Cristian
dc.contributor.authorLangie, Sabine A S
dc.contributor.authorDusinska, Maria
dc.contributor.authorAzqueta, Amaya
dc.date.accessioned2021-08-05T16:17:41Z
dc.date.available2024-08-05T00:30:20Z
dc.date.issued2021-05
dc.description.abstractDNA damage and repair activity are often assessed in blood samples from humans in different types of molecular epidemiology studies. However, it is not always feasible to analyze the samples on the day of collection without any type of storage. For instance, certain studies use repeated sampling of cells from the same subject or samples from different subjects collected at different time points, and it is desirable to analyze all these samples in the same comet assay experiment. In addition, flawless comet assay analyses on frozen samples open up the possibility of using this technique on biobank material. In this article, we discuss the use of cryopreserved peripheral blood mononuclear cells (PBMCs), buffy coat (BC), and whole blood (WB) for analysis of DNA damage and repair using the comet assay. The published literature and the authors' experiences indicate that various types of blood samples can be cryopreserved with only a minor effect on the basal level of DNA damage. There is evidence to suggest that WB and PBMCs can be cryopreserved for several years without much effect on the level of DNA damage. However, care should be taken when cryopreserving WB and BCs. It is possible to use either fresh or frozen samples of blood cells, but results from fresh and frozen cells should not be used in the same dataset. The article outlines detailed protocols for the cryopreservation of PBMCs, BCs, and WB samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMøller P, Bankoglu EE, Stopper H, Giovannelli L, Ladeira C, Koppen G, et al. Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies. Mutagenesis. 2021;36(3):193-212.pt_PT
dc.identifier.doi10.1093/mutage/geab012pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/13625
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford Academicpt_PT
dc.relation.publisherversionhttps://academic.oup.com/mutage/article/36/3/193/6182494pt_PT
dc.subjectComet assaypt_PT
dc.subjectCryopreservationpt_PT
dc.subjectDNApt_PT
dc.subjectDNA damagept_PT
dc.subjectEpidemiologypt_PT
dc.subjectMolecularpt_PT
dc.subjectLeucocytespt_PT
dc.subjectWhole bloodpt_PT
dc.titleCollection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studiespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage212pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage193pt_PT
oaire.citation.titleMutagenesispt_PT
oaire.citation.volume36pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Collection and storage of human white blood cells for analysis of DNA damage and repair activity using the comet assay in molecular epidemiology studies.pdf
Size:
666.6 KB
Format:
Adobe Portable Document Format
Download

Collections

ESTeSL - Artigos