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Spectral biomarkers of genotoxicity from methanol extracts of blood

2019-04Journal article Restricted access
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dc.contributor.authorTeixeira, Helder Paz
dc.contributor.authorRamalhete, Luís
dc.contributor.authorLadeira, Carina
dc.contributor.authorCalado, Cecilia R. C.
dc.date.accessioned2019-05-27T16:12:51Z
dc.date.available2019-05-27T16:12:51Z
dc.date.issued2019-04
dc.description.abstractIt is relevant to develop new monitoring techniques of carcinogenic risk associated with environmental exposition to genotoxic chemicals. The conventional biomonitoring techniques are based on laborious, expensive methods as the ones requiring isolation of lymphocytes from peripheral blood, in vitro cell culture, followed by e.g. cytokinesis-block assay and microscope observation of chromosomal abnormalities. The present work evaluated an infrared spectroscopy method, based on a simple, more economical and high-throughput procedure of analysis of whole blood processed with methanol. It was possible to identify ratios of spectral bands that are statistically different between hospital professionals occupationally exposed to antineoplastic drugs, such as 5-fluorouracil and non-hospital professionals without this exposure. It was also identified ratios of spectral bands which are statistically different between participants presenting lymphocytes with chromosomal abnormalities (as micronucleus, nuclear buds, and nucleoplasmatic bridges) and participants not presenting these abnormalities. The infrared spectroscopy-based method presents, therefore, appealing characteristics to be applied in more intensive and/or large-scale studies of monitoring genotoxic risks.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTeixeira HP, Ramalhete L, Ladeira C, Calado CR. Spectral biomarkers of genotoxicity from methanol extracts of blood. In: ENBENG 2019 – Proceedings of the 6th IEEE Portuguese Meeting on Bioengineering, Instituto Superior de Engenharia de Lisboa (ISEL), Portugal, February 22-23, 2019. Article no. 8692462.pt_PT
dc.identifier.doi10.1109/ENBENG.2019.8692462pt_PT
dc.identifier.isbn9781538685068
dc.identifier.urihttp://hdl.handle.net/10400.21/10076
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherIEEEpt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/document/8692462pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiomarkerspt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectInfrared spectroscopypt_PT
dc.subjectCell culturept_PT
dc.subjectHospitalpt_PT
dc.subjectLymphocytespt_PT
dc.subjectMethanolpt_PT
dc.subjectAntineoplastic drugspt_PT
dc.subjectCarcinogenic riskpt_PT
dc.subjectChromosomal abnormalitiespt_PT
dc.subjectBloodpt_PT
dc.subjectIPL/2017/GenTox_ESTeSLpt_PT
dc.titleSpectral biomarkers of genotoxicity from methanol extracts of bloodpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4pt_PT
oaire.citation.startPage1pt_PT
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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