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IL-5 serum and appendicular lavage fluid concentrations correlate with eosinophilic infiltration in the appendicular wall supporting a role for a hypersensitivity type I reaction in acute appendicitis

2022-12Journal article Open access
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dc.contributor.authorCarvalho, Nuno
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorCoelho, Hélder
dc.contributor.authorCóias, Ana
dc.contributor.authorTrindade, Madalena
dc.contributor.authorVaz, João
dc.contributor.authorCismasiu, Brigitta
dc.contributor.authorMoita, Catarina
dc.contributor.authorMoita, Luis
dc.contributor.authorCosta, Paulo Matos
dc.date.accessioned2022-12-09T12:02:42Z
dc.date.available2022-12-09T12:02:42Z
dc.date.issued2022-12
dc.description.abstractAppendicitis is the most common abdominal surgical emergency, but its aetiology is not fully understood. We and others have proposed that allergic responses play significant roles in its pathophysiology. Eosinophils and Interleukin (IL)-5 are involved in a hypersensitivity type I reaction. Eosinophil infiltration is common in the allergic target organ and is dependent on IL-5. In the presence of an allergic component, it is expected that the eosinophil count and IL-5 local and systemic concentrations become elevated. To address this hypothesis, we designed a prospective study that included 65 patients with acute appendicitis (grouped as acute phlegmonous or gangrenous according to the histological definition) and 18 patients with the clinical diagnosis of acute appendicitis, but with normal histological findings (control group) were enrolled. Eosinophil blood counts and appendicular wall eosinophil infiltration were determined. IL-5 levels in the blood and appendicular lavage fluid were evaluated. Appendicular lavage fluid was collected by a new methodology developed and standardized by our group. Appendicular wall eosinophil infiltration was higher in acute phlegmonous appendicitis than in gangrenous appendicitis (p = 0.000). IL-5 blood levels were similar in both pathologic and control groups (p > 0.05). In the appendicular lavage fluid, higher levels of IL-5 were observed in the phlegmonous appendicitis group (p = 0.056). We found a positive correlation between the appendicular wall eosinophilic infiltration and the IL-5 concentrations, in both the blood and the appendicular lavage fluid, supporting the IL-5 reliance in eosinophil local infiltration. We observed the highest presence of eosinophils at phlegmonous appendicitis walls. In conclusion, the present data are compatible with a hypersensitivity type I allergic reaction in the target organ, the appendix, during the phlegmonous phase of appendicitis.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarvalho N, Carolino E, Coelho H, Cóias A, Trindade M, Vaz J, et al. IL-5 serum and appendicular lavage fluid concentrations correlate with eosinophilic infiltration in the appendicular wall supporting a role for a hypersensitivity type I reaction in acute appendicitis. Int J Mol Sci. 2022;23(23):15086.pt_PT
dc.identifier.doi10.3390/ijms232315086pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15135
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/23/23/15086pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAllergypt_PT
dc.subjectAppendicitispt_PT
dc.subjectAppendicular lavage fluidpt_PT
dc.subjectEosinophilspt_PT
dc.subjectIL-5pt_PT
dc.subjectTh2 cytokinept_PT
dc.titleIL-5 serum and appendicular lavage fluid concentrations correlate with eosinophilic infiltration in the appendicular wall supporting a role for a hypersensitivity type I reaction in acute appendicitispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue23pt_PT
oaire.citation.startPage15086pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
person.familyNameCarolino
person.givenNameElisabete
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.orcid0000-0003-4165-7052
person.identifier.ridF-1012-2015
person.identifier.scopus-author-id25821697000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication.latestForDiscovery77930d39-ed34-44dc-a4a6-9bf833e5e688

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