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A proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic activity of γ‐conglutin from Lupinus albus seeds

2021-09Artigo científico Acesso aberto
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dc.contributor.authorGrácio, Madalena
dc.contributor.authorRocha, João
dc.contributor.authorPinto, Rui
dc.contributor.authorBoavida Ferreira, Ricardo
dc.contributor.authorSolas, João
dc.contributor.authorEduardo‐Figueira, Maria
dc.contributor.authorSepodes, Bruno
dc.contributor.authorRibeiro, Ana Cristina
dc.date.accessioned2021-10-12T11:02:20Z
dc.date.available2021-10-12T11:02:20Z
dc.date.issued2021-09
dc.description.abstractExperiments conducted in vitro and in vivo, as well as clinical trials for hypoglycemic therapeutics, support the hypoglycemic properties of the lectin γ-conglutin, a Lupinus seed storage protein, by a mechanism not yet been clarified. Structural studies established that binding of γ-conglutin, in native and denatured form, to insulin occurs by a strong binding that resists rupture when 0.4 M NaCl and 0.4 M galactose are present, suggesting that strong electrostatic interactions are involved. Studies on the binding of γ-conglutin in the native and denatured form to HepG2 membrane glycosylated receptors were conducted, which reveal that only the native form of γ-conglutin with lectin activity is capable of binding to these receptors. Glycosylated insulin receptors were detected on purified HepG2 cell membranes and characterized by 1D and 2D analyses. Preclinical assays with male mice (CD-1) indicated that native and denatured γ-conglutinates display an antihyperglycemic effect, decreasing glucose in blood comparable after 120 min to that exhibited by the animal group treated with metformin, used to treat T2D and used as a positive control. Measurement of organ injury/functional biomarkers (hepatic, pancreatic, renal, and lipid profile) was comparable to that of metformin treatment or even better in terms of safety endpoints (pancreatic and hepatic biomarkers).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGracio M, Rocha J, Pinto R, Ferreira RB, Solas J, Eduardo-Figueira M, et al. A proposed lectin-mediated mechanism to explain the in vivo antihyperglycemic activity of gamma-conglutin from Lupinus albus seeds. Food Sci Nutr. 2021;9(11):5980-96.pt_PT
dc.identifier.doi10.1002/fsn3.2520pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/13866
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1002/fsn3.2520pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDiabetes mellitus type 2pt_PT
dc.subjectHypoglycemic activitypt_PT
dc.subjectInsulin receptorspt_PT
dc.subjectLectinspt_PT
dc.subjectGamma-conglutinpt_PT
dc.titleA proposed lectin‐mediated mechanism to explain the in Vivo antihyperglycemic activity of γ‐conglutin from Lupinus albus seedspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage5996pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage5980pt_PT
oaire.citation.titleFood Science & Nutritionpt_PT
oaire.citation.volume9pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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