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Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy

2013-05-23Conference object Open access
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dc.contributor.authorLopes, Marta B.
dc.contributor.authorSales, Kevin C.
dc.contributor.authorLopes, Vítor V.
dc.contributor.authorCalado, Cecília
dc.date.accessioned2020-07-30T14:20:08Z
dc.date.available2020-07-30T14:20:08Z
dc.date.issued2013-05-23
dc.description.abstractThe development of in-situ monitoring techniques enabling the real-time acquisition of information concerning the key variables over different Escherichia coli cultivation conditions and strategies is a crucial step towards the optimization of a plasmid bioproduction process. This work shows the use of a Near-InfraRed (NIR) fiber optic probe immersed in the culture broth for the real-time acquisition of NIR spectra along different E. coli cultures conducted with mixtures of the carbon sources glucose and glycerol, and performed in both batch and fed-batch modes. Accurate partial least squares models based on the acquired spectral data over such different cultivation conditions were built, yielding a R 2 = 0.97 for biomass and plasmid productions and a RMSEP of 0.34 and 7.52, respectively; a R 2 of 0.93 and a RMSEP of 0.46 and 0.33 was obtained for glucose and glycerol, respectively; the acetate model produced a R 2 of 0.96 and a RMSEP of 0.32.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationLOPES, Marta B.; [et al] – Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy. In 2013 IEEE 3rd Portuguese Meeting in Bioengineering (ENBENG). Braga, Portugal: IEEE, 2013. ISBN 978-1-4673-4859-1. Pp. 1-4pt_PT
dc.identifier.doi10.1109/ENBENG.2013.6518394pt_PT
dc.identifier.isbn978-1-4673-4859-1
dc.identifier.urihttp://hdl.handle.net/10400.21/12119
dc.language.isoengpt_PT
dc.publisherInstitute of Electrical and Electronics Engineerspt_PT
dc.relationPTDC/BI0/6924212006 - FCTpt_PT
dc.relationSFRH/BPDI73758/201O - FCTpt_PT
dc.relation.publisherversionhttps://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6518394pt_PT
dc.subjectPlasmidpt_PT
dc.subjectMonitoringpt_PT
dc.subjectControlpt_PT
dc.subjectFourier transform infraredpt_PT
dc.subjectNear-infraredpt_PT
dc.subjectSpectroscopypt_PT
dc.subjectEscherichia colipt_PT
dc.titleReal-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlace20-23 Feb. 2013 - Braga, Portugalpt_PT
oaire.citation.endPage4pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.title2013 IEEE 3rd Portuguese Meeting in Bioengineering (ENBENG)pt_PT
person.familyNameB. Lopes
person.familyNameCalado
person.givenNameMarta
person.givenNameCecília
person.identifier1195979
person.identifier130332
person.identifier.ciencia-idFD16-A07F-7B12
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-4135-1857
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridF-5378-2011
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id55489480400
person.identifier.scopus-author-id6603163260
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication183b936b-4a1d-4c80-9405-17491abb3d64
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscoverye8577257-c64c-4481-9b2b-940fedb360cc

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