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Advisor(s)
Abstract(s)
The development of in-situ monitoring techniques enabling the real-time acquisition of information concerning the key variables over different Escherichia coli cultivation conditions and strategies is a crucial step towards the optimization of a plasmid bioproduction process. This work shows the use of a Near-InfraRed (NIR) fiber optic probe immersed in the culture broth for the real-time acquisition of NIR spectra along different E. coli cultures conducted with mixtures of the carbon sources glucose and glycerol, and performed in both batch and fed-batch modes. Accurate partial least squares models based on the acquired spectral data over such different cultivation conditions were built, yielding a R 2 = 0.97 for biomass and plasmid productions and a RMSEP of 0.34 and 7.52, respectively; a R 2 of 0.93 and a RMSEP of 0.46 and 0.33 was obtained for glucose and glycerol, respectively; the acetate model produced a R 2 of 0.96 and a RMSEP of 0.32.
Description
Keywords
Plasmid Monitoring Control Fourier transform infrared Near-infrared Spectroscopy Escherichia coli
Citation
LOPES, Marta B.; [et al] – Real-time plasmid monitoring of batch and fed-batch Escherichia coli cultures by NIR spectroscopy. In 2013 IEEE 3rd Portuguese Meeting in Bioengineering (ENBENG). Braga, Portugal: IEEE, 2013. ISBN 978-1-4673-4859-1. Pp. 1-4
Publisher
Institute of Electrical and Electronics Engineers