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Discriminating B and T-lymphocyte from its molecular profile acquired in a label-free and high-throughput method

2020-11Artigo científico Acesso restrito
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dc.contributor.authorRamalhete, Luís
dc.contributor.authorAraújo, Rúben
dc.contributor.authorCalado, Cecília
dc.date.accessioned2020-10-30T10:26:28Z
dc.date.available2020-10-30T10:26:28Z
dc.date.issued2020-11
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2018 do Instituto Politécnico de Lisboa. Código de referência IPL/2018/RENALPROG_ISEL
dc.descriptionEste trabalho foi financiado pelo Concurso Anual para Projetos de Investigação, Desenvolvimento, Inovação e Criação Artística (IDI&CA) 2020 do Instituto Politécnico de Lisboa. Código de referência IPL/2020/NephroMD:ISEL
dc.description.abstractB and T-lymphocytes are one of the main players of the adaptive immune system and consequently, the ability to discriminate these cells is relevant in diverse pathophysiological cases. In the present work, FTIR spectroscopy was used to acquire the cells molecular profile in a label-free, simple, rapid, economic, and high-throughput mode. It was possible, by hierarchical cluster analysis of the second derivative spectra, between 900 to 1500 cm-1, to correctly classify 98% of samples as B and T-lymphocyte. It was also possible to develop a very good support vector machine model, which could predict B and T-lymphocyte from the second derivative spectra, with high accuracy (0.95), precision (0.95), recall (0.95), and F1 score (0.95). The method developed presents therefore, appealing characteristics to promote a new diagnostic tool to analyze and discriminate B from T-lymphocytes.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationRAMALHETE, Luís; ARAÚJO, Rúben; CALADO, Cecília R. C. – Discriminating B and T-lymphocyte from its molecular profile acquired in a label-free and high-throughput method. Vibrational Spectroscopy. ISSN 0924-2031. Vol. 111 (2020), pp. 1-8pt_PT
dc.identifier.doi10.1016/j.vibspec.2020.103177pt_PT
dc.identifier.issn0924-2031
dc.identifier.urihttp://hdl.handle.net/10400.21/12305
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2018/RENALPROG_ISELpt_PT
dc.relationProjeto financiado no âmbito do Concurso de Projetos de Investigação, Desenvolvimento, Inovação & Criação Artística (IDI&CA) financiados pelo Instituto Politécnico de Lisboa. IPL/2020/NephroMD:ISELpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S0924203120301818?token=673747434D51FE4936C95BEB2B0D3908C7B764FEE7DDDCCF688F8A6F7E1E4DCD173C3D58DA40EEF3C7EA1A78CBA0B174pt_PT
dc.subjectB lymphocytespt_PT
dc.subjectT-lymphocytespt_PT
dc.subjectMolecular profilept_PT
dc.subjectFTIR spectroscopypt_PT
dc.titleDiscriminating B and T-lymphocyte from its molecular profile acquired in a label-free and high-throughput methodpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage8pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleVibrational Spectroscopypt_PT
oaire.citation.volume111pt_PT
person.familyNameRamalhete
person.familyNameAraújo
person.familyNameCalado
person.givenNameLuís
person.givenNameRúben Alexandre Dinis
person.givenNameCecília
person.identifier2296066
person.identifier130332
person.identifier.ciencia-idDF19-022D-AA10
person.identifier.ciencia-id9A18-BFDC-ED95
person.identifier.ciencia-id9418-E320-3177
person.identifier.orcid0000-0002-8911-3380
person.identifier.orcid0000-0002-9369-6486
person.identifier.orcid0000-0002-5264-9755
person.identifier.ridE-2102-2014
person.identifier.scopus-author-id57208672678
person.identifier.scopus-author-id6603163260
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication7846e088-851e-47b6-a7bd-d02c9d8f047d
relation.isAuthorOfPublication9998e940-5e65-4661-8308-afcb56d5df01
relation.isAuthorOfPublicatione8577257-c64c-4481-9b2b-940fedb360cc
relation.isAuthorOfPublication.latestForDiscovery7846e088-851e-47b6-a7bd-d02c9d8f047d

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ISEL - Eng. Quim. Biol. - Artigos