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The gut microbiome and hydroxyurea effect on sickle cell disease children from Angola

2023-04Conference object Open access
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dc.contributor.authorBrito, Miguel
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorGinete, Catarina
dc.contributor.authorSantos, Brígida
dc.contributor.authorVasconcelos, J.
dc.date.accessioned2023-08-25T11:36:56Z
dc.date.available2023-08-25T11:36:56Z
dc.date.issued2023-04
dc.descriptionThis work was supported by FCT/Aga Khan (project nº330842553) and FCT/MCTES (UIDB/05608/2020 and UIDP/05608/2020) – H&TRC.pt_PT
dc.description.abstractBackground: Sickle cell disease (SCD) is an inherited hematological disorder and a serious global health problem, affecting between 20 and 25 million people worldwide. In Sub-Saharan Africa, where it is more prevalent, it contributes up to 90% of under-5 mortality. Although hydroxyurea (HU) is the leading treatment for these patients, its effects on the gut microbiome have not yet been explored. Some studies reported that gastroenteritis events were less frequent in SCA children taking HU and it also significantly improved the survival from pneumococcal infections. HU may have a protective effect, not only by improving several hematological parameters but also by lowering the risk of some bacterial infections. Aims: In this context, the aim of this study was to investigate this association by characterizing the gut microbiome of an Angolan SCA pediatric population before and after 6 months of HU treatment and comparing it with a control group of healthy siblings. Results: A total of 113 fecal samples were obtained and sequenced by NGS for the 16S rRNA gene (V3-V4 regions), which corresponded to 40 children in the control and before HU groups and 33 after HU, aged between 4-12 years old. Our findings revealed that these three groups exhibit some notable differences, especially within Lachnospiraceae and Ruminococcaceae family. After HU treatment there was an increase of several beneficial bacteria, such as: Blautia coccoides (p=0.009), Blautia luti (p<0.001), Blautia faecis (p=0.008), Bifidobacterium longum (p=0.011), Dorea formicigenerans (p<0.001), Dorea massiliensis (p=0.003), Eubacterium halii (p=0.004), Elusimicrobium spp (p=0.032), Ruminococcus callidus (p=0.037), Ruminococcus faecis (p=0.012), Roseburia spp (p=0.050) and Subdoligranulum variabile (p=0.009). Most of those OTUs are SCFAs producing species, having butyrate or propionate as end-products of bacterial metabolism, both exhibiting anti-inflammatory properties. Moreover, children before HU had a higher abundance of bacteria considered pathogenic, like E. coli (p=0.001), Clostridiun_g24 (p=0.039), and H. influenzae (p=0.050). Conclusion: Overall, this study provides the first evidence of the HU effect on the gut microbiome and provides a rationale for further research for developing treatments to reduce gut microbiota-driven inflammation, which may attenuate the dysbiosis and chronic symptoms experienced by these patients.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBrito M, Delgadinho M, Ginete C, Santos B, Vasconcelos J. The gut microbiome and hydroxyurea effect on sickle cell disease children from Angola. In: Abstract Book for the 17th Annual Sickle Cell and Thalassaemia Conference. HemaSphere. 2023;7(Suppl 1):1.pt_PT
dc.identifier.doi10.1097/01.HS9.0000928132.39710.c7pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/16410
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherLippincott Williams & Wilkinspt_PT
dc.relationAga Khan_project nº 330842553pt_PT
dc.relation.publisherversionhttps://journals.lww.com/hemasphere/fulltext/2023/04001/5595849_the_gut_microbiome_and_hydroxyurea_effect.1.aspxpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectSickle cell diseasept_PT
dc.subjectGut microbiomept_PT
dc.subjectChildrenpt_PT
dc.subjectAngolapt_PT
dc.subjectAga Khan_project nº 330842553pt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleThe gut microbiome and hydroxyurea effect on sickle cell disease children from Angolapt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.endPage1pt_PT
oaire.citation.issueS1pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleHemaSpherept_PT
oaire.citation.volume7pt_PT
person.familyNameBrito
person.familyNameNeves Delgadinho
person.familyNameHonrado Ginete
person.givenNameMiguel
person.givenNameMariana Isabel
person.givenNameAna Catarina
person.identifierCAJ-5082-2022
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id8715-F62E-1E0F
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0002-2334-782X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
relation.isAuthorOfPublicationca55aab6-9a58-4f79-ab79-20513414099f
relation.isAuthorOfPublicationdfb2fbba-17ff-42fb-905a-fcfc8f326e1c
relation.isAuthorOfPublication.latestForDiscoveryca55aab6-9a58-4f79-ab79-20513414099f

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