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The hypocholesterolemic potential of the edible algae fucus vesiculosus: Proteomic and quantitative PCR analysis

2023-07-20Research article Open access
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datacite.subject.fosEngenharia e Tecnologia::Engenharia Química
dc.contributor.authorAndré, Rebeca
dc.contributor.authorPacheco, Rita
dc.contributor.authorAlves, Ana Catarina
dc.contributor.authorSantos, Hugo M. M.
dc.contributor.authorBourbon, Mafalda
dc.contributor.authorSerralheiro, Maria Luisa
dc.date.accessioned2025-09-08T12:32:28Z
dc.date.available2025-09-08T12:32:28Z
dc.date.issued2023-07-20
dc.description.abstractA brown seaweed consumed worldwide, Fucus vesiculosus, has been used to prevent atherosclerosis and hypercholesterolemia, among other uses. However, the mechanisms of action that lead to these effects are not yet fully understood. This work aims to study the in vitro effect of an aqueous extract of F. vesiculosus, previously characterized as rich in phlorotannins and peptides, on the expression of different proteins involved in the synthesis and transport of cholesterol. A proteomic analysis, Western blot, and qRT-PCR analysis were performed to identify protein changes in HepG2 cells exposed to 0.25 mg/mL of the F. vesiculosus extract for 24 h. The proteomic results demonstrated that, in liver cells, the extract decreases the expression of four proteins involved in the cholesterol biosynthesis process (CYP51A1, DHCR24, HMGCS1 and HSD17B7). Additionally, a 12.76% and 18.40% decrease in the expression of two important transporters proteins of cholesterol, NPC1L1 and ABCG5, respectively, was also observed, as well as a 30% decrease in NPC1L1 mRNA levels in the cells exposed to the extract compared to control cells. Our study reveals some of the mechanisms underlying the actions of bioactive compounds from F. vesiculosus that may explain its previously reported hypocholesterolemic effect, future prospecting its use as a functional food.eng
dc.description.sponsorshipIPL/2019/AMar2Fun - Instituto Politécnico de Lisboa
dc.identifier.citationAndré, R., Pacheco, R., Alves, A. C., Santos, H. M. M., Bourbon, M., & Serralheiro, M. L. (2023). The hypocholesterolemic potential of the edible algae fucus vesiculosus: Proteomic and quantitative PCR analysis. Foods, 12(14), 1-14. https://doi.org/10.3390/foods12142758
dc.identifier.doihttps://doi.org/10.3390/foods12142758
dc.identifier.eissn2304-8158
dc.identifier.urihttp://hdl.handle.net/10400.21/22125
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationBiosystems and Integrative Sciences Institute
dc.relationCentro de Química Estrutural
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationPTDC/BIA-BQM/28355/2017
dc.relationLA/P/0056/2020
dc.relationPD/BD/142861/2018
dc.relation.hasversionhttps://www.mdpi.com/2304-8158/12/14/2758
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectFucus vesiculosus
dc.subjectCholesterol synthesis
dc.subjectCholesterol excretion
dc.subjectEzetimibe
dc.subjectNPC1L1
dc.subjectABCG5
dc.titleThe hypocholesterolemic potential of the edible algae fucus vesiculosus: Proteomic and quantitative PCR analysiseng
dc.typeresearch article
dspace.entity.typePublication
oaire.awardTitleBiosystems and Integrative Sciences Institute
oaire.awardTitleCentro de Química Estrutural
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04046%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00100%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.endPage14
oaire.citation.issue14
oaire.citation.startPage1
oaire.citation.titleFoods
oaire.citation.volume12
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43
person.familyNameAndré
person.familyNamePacheco
person.familyNameBourbon
person.familyNameSerralheiro
person.givenNameRebeca
person.givenNameRita
person.givenNameMafalda
person.givenNameMaria Luisa
person.identifier1173301
person.identifier1022927
person.identifier.ciencia-id7D18-8206-8EF7
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.ciencia-id4B19-52C6-8C6F
person.identifier.orcid0000-0002-2733-5797
person.identifier.orcid0000-0001-5192-3006
person.identifier.orcid0000-0001-8843-3799
person.identifier.orcid0000-0001-7541-9613
person.identifier.ridC-3062-2012
person.identifier.ridE-3407-2012
person.identifier.scopus-author-id57207778987
person.identifier.scopus-author-id8853708300
person.identifier.scopus-author-id6602688573
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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