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Design and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesicles

2020-05Conference object Restricted access
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dc.contributor.authorFernandes, Ana
dc.contributor.authorRosa, Sara
dc.contributor.authorSilva, Ricardo
dc.contributor.authorCunha, Raquel
dc.contributor.authorFuzeta, Miguel Almeida
dc.contributor.authorCalado, Cecília
dc.contributor.authorCarvalho, Carla
dc.contributor.authorCabral, Joaquim M.S.
dc.contributor.authorAzevedo, Ana
dc.date.accessioned2022-03-08T11:54:49Z
dc.date.available2022-03-08T11:54:49Z
dc.date.issued2020-05
dc.description.abstractCurrently, the production of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSC) is performed in laborious and time-consuming static culture systems using serum-containing media, and their purification is through ultracentrifugation based methods. The whole process lacks on resolution, selectivity and capacity. Additionally, several differences were observed, in terms of the cargo of EVs, between EVs isolated from culture supernatants of MSC expanded under different culture conditions, stressing the importance of controlling all culture process parameters. A robust and indepth process to manufacture MSC-EVs may provide a new therapeutic paradigm for cell-free MSC-based therapies.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFERNANDES-PLATZGUMMER, Ana; [et al] – Design and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesicles. (2020 Meeting of ISCT-International Society for Cell & Gene Therapy, 28-29th May, Paris). Cytotherapy. 22(5), Suplement, May 2020, pp. S48-s49.pt_PT
dc.identifier.doi10.1016/j.jcyt.2020.03.058pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/14402
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1465324920301225#!pt_PT
dc.subjectExtracellular vesicles (EVs)pt_PT
dc.subjectMesenchymal stromal cells (MSC)pt_PT
dc.titleDesign and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesiclespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.endPageS49pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPageS48pt_PT
oaire.citation.titleCytotherapypt_PT
oaire.citation.volume22pt_PT
person.familyNameFernandes-Platzgummer
person.familyNameda Silva Lourenço Rosa
person.familyNameCalado
person.familyNameCabral
person.givenNameAna
person.givenNameSara Alexandra
person.givenNameCecília
person.givenNameJoaquim M.S.
person.identifier657490
person.identifier130332
person.identifier.ciencia-idEA11-6777-F573
person.identifier.ciencia-idAF16-13F9-CA88
person.identifier.ciencia-id9418-E320-3177
person.identifier.ciencia-idAB13-9ED3-C821
person.identifier.orcid0000-0002-5367-6528
person.identifier.orcid0000-0002-3331-2671
person.identifier.orcid0000-0002-5264-9755
person.identifier.orcid0000-0002-2405-5845
person.identifier.ridF-1685-2011
person.identifier.ridE-2102-2014
person.identifier.ridG-2052-2010
person.identifier.scopus-author-id35749894500
person.identifier.scopus-author-id6603163260
person.identifier.scopus-author-id7201350203
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicatione9ec518c-41f4-4d7a-ad45-6eb2fae83693
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relation.isAuthorOfPublication.latestForDiscoverye9ec518c-41f4-4d7a-ad45-6eb2fae83693

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