Design and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesicles

Fernandes, Ana; Rosa, Sara; Silva, Ricardo; Cunha, Raquel; Fuzeta, Miguel Almeida; Calado, Cecília; Carvalho, Carla; Cabral, Joaquim M.S.; Azevedo, Ana

http://hdl.handle.net/10400.21/14402

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Authors

Fuzeta, Miguel Almeida

Abstract(s)

Currently, the production of extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSC) is performed in laborious and time-consuming static culture systems using serum-containing media, and their purification is through ultracentrifugation based methods. The whole process lacks on resolution, selectivity and capacity. Additionally, several differences were observed, in terms of the cargo of EVs, between EVs isolated from culture supernatants of MSC expanded under different culture conditions, stressing the importance of controlling all culture process parameters. A robust and indepth process to manufacture MSC-EVs may provide a new therapeutic paradigm for cell-free MSC-based therapies.

Keywords

Extracellular vesicles (EVs) Mesenchymal stromal cells (MSC)

URI

http://hdl.handle.net/10400.21/14402

Citation

FERNANDES-PLATZGUMMER, Ana; [et al] – Design and operation of a fully controlled platform for the production and purification of well-defined mesenchymal stromal cell (MSC)-derived extracellular vesicles. (2020 Meeting of ISCT-International Society for Cell & Gene Therapy, 28-29th May, Paris). Cytotherapy. 22(5), Suplement, May 2020, pp. S48-s49.