Repository logo
 
Publication

Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells

2010-08Journal article Open access
Simple item page
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorCorreia, Daniel V.
dc.contributor.authorGrosso, Ana R.
dc.contributor.authorLanca, Telma
dc.contributor.authorFerreira, Cristina
dc.contributor.authorLacerda, João F.
dc.contributor.authorBarata, João T.
dc.contributor.authorSilva, Maria Gomes da
dc.contributor.authorSilva-Santos, Bruno
dc.date.accessioned2020-08-26T15:30:17Z
dc.date.available2020-08-26T15:30:17Z
dc.date.issued2010-08
dc.description.abstractBackground: Vγ9Vδ2 T lymphocytes are regarded as promising mediators of cancer immunotherapy due to their capacity to eliminate multiple experimental tumors, particularly within those of hematopoietic origin. However, Vγ9Vδ2 T-cell based lymphoma clinical trials have suffered from the lack of biomarkers that can be used as prognostic of therapeutic success. Design and Methods: We have conducted a comprehensive study of gene expression in acute lymphoblastic leukemia and non-Hodgkin’s lymphomas, aimed at identifying markers of susceptibility versus resistance to Vγ9Vδ2 T cell-mediated cytotoxicity. We employed cDNA microarrays and quantitative real-time PCR to screen 20 leukemia and lymphoma cell lines, and 23 primary hematopoietic tumor samples. These data were analyzed using state-of-the-art bioinformatics, and gene expression patterns were correlated with susceptibility to Vγ9Vδ2 T cell-mediated cytolysis in vitro. Results: We identified a panel of 10 genes encoding cell surface proteins that were statistically differentially expressed between “γδ-susceptible” and “γδ-resistant” hematopoietic tumors. Within this panel, 3 genes (ULBP1, TFR2, and IFITM1) were associated with increased susceptibility to Vγ9Vδ2 T-cell cytotoxicity, whereas the other 7 (CLEC2D, NRP2, SELL, PKD2, KCNK12, ITGA6, and SLAMF1) were enriched in resistant tumors. Furthermore, some of these candidates displayed a striking variance of expression among primary follicular lymphomas and T-cell acute lymphoblastic leukemias. Conclusions: Our results suggest that hematopoietic tumors display a highly variable repertoire of surface proteins that can impact on Vγ9Vδ2 cell-mediated immune targeting. The prognostic value of the proposed markers can now be evaluated in the upcoming Vγ9Vδ2 T cell-based lymphoma/leukemia clinical trials.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGomes AQ, Correia DV, Grosso AR, Lança T, Ferreira C, Lacerda JF, et al. dentification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells. Haematologica. 2010;95(8):1397-404.pt_PT
dc.identifier.doi10.3324/haematol.2009.020602pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12196
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFerrata Storti Foundationpt_PT
dc.relation.publisherversionhttp://www.haematologica.org/content/95/8/1397pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectAntigens, Differentiationpt_PT
dc.subjectAntigens, Surfacept_PT
dc.subjectCell line, Tumorpt_PT
dc.subjectCells, Culturedpt_PT
dc.subjectCytotoxicity, Immunologicpt_PT
dc.subjectGPI-linked proteinspt_PT
dc.subjectGenetic predisposition to diseasept_PT
dc.subjectHumanspt_PT
dc.subjectIntracellular signaling peptides and proteinspt_PT
dc.subjectJurkat cellspt_PT
dc.subjectLeukemiapt_PT
dc.subjectLeukocytes, Mononuclearpt_PT
dc.subjectLymphomapt_PT
dc.subjectMembrane Proteinspt_PT
dc.subjectOligonucleotide array sequence analysispt_PT
dc.subjectPrognosispt_PT
dc.subjectReceptors, Antigenpt_PT
dc.subjectReceptors, Transferrinpt_PT
dc.subjectReverse transcriptase polymerase chain reactionpt_PT
dc.subjectT-Lymphocytespt_PT
dc.subjectGene expression profilingpt_PT
dc.subjectT-Cellpt_PT
dc.subjectGamma-deltapt_PT
dc.titleIdentification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1404pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1397pt_PT
oaire.citation.titleHaematologicapt_PT
oaire.citation.volume95pt_PT
person.familyNameGomes
person.givenNameAnita
person.identifier.ciencia-id4B10-E015-52B7
person.identifier.orcid0000-0002-3348-0448
person.identifier.ridC-3580-2014
person.identifier.scopus-author-id7202386033
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875
relation.isAuthorOfPublication.latestForDiscovery2b5a3f0a-29c2-4ecd-a83f-50d0b99a4875

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells.pdf
Size:
479.62 KB
Format:
Adobe Portable Document Format
Download

Collections

ESTeSL - Artigos