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Mechanism of action and the biological activities of Nigella sativa oil components

2020-12Journal article Restricted access
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dc.contributor.authorSilva, André Filipe C.
dc.contributor.authorHaris, Parvez I.
dc.contributor.authorSerralheiro, Maria Luisa
dc.contributor.authorPacheco, Rita
dc.date.accessioned2021-01-15T11:40:33Z
dc.date.available2021-01-15T11:40:33Z
dc.date.issued2020-12
dc.description.abstractNigella sativa seeds oil is consumed because of its health benefits. Although the oil has been studied for its properties and composition, these effects are often associated with its major compound thymoquinone, and less has been reported about the other components' contributions to the pharmacological properties and mechanism of action. The present study aimed to evaluate the oil compounds' antioxidant and anti-tumour activities, and its effect on molecular targets associated with the claimed health effects. A wide a range of biochemical and biophysical techniques were used to understand the biological activities of the oil components. Compounds such as thymoquinone and volatile monoterpenes such as alpha-thujene, alpha-pynene, sabinene and 3-carene were identified by using GC-MS. Similar high antioxidant activities were obtained for the oil and its non-volatile (NV) fraction, with enzymes inhibition and cytotoxic activity against MCF-7 and A375 cells the oil showed a strong effect but not its NV fraction. Analysis of A375 cells' proteome in contact with the oil showed increased expression of proteins POTEF and HSP 90-b suggesting that the oil's antitumor effect was due to apoptosis and cellular stress. FT-IR studies in two model proteins in contact with the oil allowed oil compounds-protein interactions to be recognized and also showed that volatiles stabilized proteins. Therefore, not only thymoquinone but also the volatile monoterpenes were important for the diversity of properties, and therefore modifications in the chemical composition of the oil, as an outcome of storage or even food preparation, may affect its biological activity.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSILVA, André Filipe C.; [et al] – Mechanism of action and the biological activities of Nigella sativa oil componentes. Food Bioscience. ISSN 2212-4292. Vol. 38 (2020), pp. 1-11pt_PT
dc.identifier.doi10.1016/j.fbio.2020.100783pt_PT
dc.identifier.eissn2212-4306
dc.identifier.issn2212-4292
dc.identifier.urihttp://hdl.handle.net/10400.21/12611
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationUID/MULTI/04046/2019 - FCTpt_PT
dc.relationPTDC/BIA-BQM/28355/2017 - FCTpt_PT
dc.relation.publisherversionhttps://reader.elsevier.com/reader/sd/pii/S2212429220311214?token=71BCCE8AC5C6F9CDAE6495A8FFA44DC34B49557482C33B70CE6989920B0EA9EBE90D0C9D389E81321C72DB7390B0CFFApt_PT
dc.subjectNigella sativa seed oilpt_PT
dc.subjectBlack cuminpt_PT
dc.subjectKalonji seedpt_PT
dc.subjectThymoquinonept_PT
dc.subjectMCF-7pt_PT
dc.subjectA375pt_PT
dc.titleMechanism of action and the biological activities of Nigella sativa oil componentspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage11pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleFood Biosciencept_PT
oaire.citation.volume38pt_PT
person.familyNameSerralheiro
person.familyNamePacheco
person.givenNameMaria Luisa
person.givenNameRita
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0001-7541-9613
person.identifier.orcid0000-0001-5192-3006
person.identifier.ridE-3407-2012
person.identifier.ridC-3062-2012
person.identifier.scopus-author-id6602688573
person.identifier.scopus-author-id8853708300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication88895442-2a46-4878-a83f-435152a9cbb4
relation.isAuthorOfPublicationd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isAuthorOfPublication.latestForDiscoveryd6907c0a-3f8f-431c-acf2-0b257692f0d4

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