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Inhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leaves

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dc.contributor.authorArantes, Ana A.
dc.contributor.authorFale, Pedro L.
dc.contributor.authorCosta, Larissa C. B.
dc.contributor.authorPacheco, Rita
dc.contributor.authorAscensão, Lia
dc.contributor.authorSerralheiro, Maria Luísa
dc.date.accessioned2017-11-27T15:42:46Z
dc.date.available2017-11-27T15:42:46Z
dc.date.issued2016
dc.description.abstractThe aim ofthis study was to provide scientific knowledge to supportthe use of Vernonia condensata Baker, Asteraceae, beverages for their alleged hypocholesterolemic properties by testing their action as HMGCoA reductase inhibitors and their capacity to lower dietary cholesterol permeation. Chlorogenic acid, and other caffeoylquinic acids derivatives were identified as the main components of these beverages by LC–MS/MS. No changes in the composition were notice after the in vitro gastrointestinal digestion and no toxicity against Caco-2 and HepG2 cell lines was detected. Cholesterol permeation through Caco-2 monolayers was reduced in 37% in the presence of these herbal teas, and the caffeoylquinic acids permeated the monolayers in 30–40% of their initial amount in 6 h. HMG-CoA reductase activity was reduced with these beverages, showing an IC50 of 217 g ml−1. It was concluded that caffeoylquinic acids, the major components, justified 98% of the enzyme inhibition measured.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationARANTES, Ana A.; [et al] - Inhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leaves. Revista Brasileira de Farmacognosia. ISSN 0102-695X. Vol. 26, N. º 6 (2016), pp. 738-743pt_PT
dc.identifier.doi10.1016/j.bjp.2016.05.008pt_PT
dc.identifier.issn0102-695X
dc.identifier.urihttp://hdl.handle.net/10400.21/7594
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSociedade Brasileira de Farmocognosiapt_PT
dc.relationMULTI/00612pt_PT
dc.relation.publisherversionhttps://ac.els-cdn.com/S0102695X16301065/1-s2.0-S0102695X16301065-main.pdf?_tid=045b60f0-d388-11e7-9fe8-00000aacb360&acdnat=1511796860_5267543bdf4a80388a8c429b1c5ca9a2pt_PT
dc.subjectVernonia condensatapt_PT
dc.subjectHMG-CoA reductasept_PT
dc.subjectChlorogenic acidpt_PT
dc.subjectCynarinpt_PT
dc.subjectCholesterolpt_PT
dc.subjectCaco-2 cellspt_PT
dc.titleInhibition of HMG-CoA reductase activity and cholesterol permeation through Caco-2 cells by caffeoylquinic acids from Vernonia condensata leavespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.citation.endPage743pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage738pt_PT
oaire.citation.titleRevista Brasileira de Farmacognosiapt_PT
oaire.citation.volume26pt_PT
oaire.fundingStream5876
person.familyNamePacheco
person.givenNameRita
person.identifier1022927
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0001-5192-3006
person.identifier.ridC-3062-2012
person.identifier.scopus-author-id8853708300
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isAuthorOfPublication.latestForDiscoveryd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isProjectOfPublication6b231382-2ec4-46c9-a9b5-51699bd7cab5
relation.isProjectOfPublication.latestForDiscovery6b231382-2ec4-46c9-a9b5-51699bd7cab5

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