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Occluding junctions as novel regulators of tissue mechanics during wound repair

2018-12Journal article Restricted access
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dc.contributor.authorCarvalho, Lara
dc.contributor.authorPatricio, Pedro
dc.contributor.authorPonte, Susana
dc.contributor.authorHeisenberg, Carl-Philipp
dc.contributor.authorAlmeida, Luís
dc.contributor.authorNunes, André S.
dc.contributor.authorAraujo, Nuno
dc.contributor.authorJacinto, Antonio
dc.date.accessioned2019-04-10T13:17:18Z
dc.date.available2019-04-10T13:17:18Z
dc.date.issued2018-12
dc.description.abstractIn epithelial tissues, cells tightly connect to each other through cell-cell junctions, but they also present the remarkable capacity of reorganizing themselves without compromising tissue integrity. Upon injury, simple epithelia efficiently resolve small lesions through the action of actin cytoskeleton contractile structures at the wound edge and cellular rearrangements. However, the underlying mechanisms and how they cooperate are still poorly understood. In this study, we combine live imaging and theoretical modeling to reveal a novel and indispensable role for occluding junctions (OJs) in this process. We demonstrate that OJ loss of function leads to defects in wound-closure dynamics: instead of contracting, wounds dramatically increase their area. OJ mutants exhibit phenotypes in cell shape, cellular rearrangements, and mechanical properties as well as in actin cytoskeleton dynamics at the wound edge. We propose that OJs are essential for wound closure by impacting on epithelial mechanics at the tissue level, which in turn is crucial for correct regulation of the cellular events occurring at the wound edge.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCARVALHO, Lara; [et al] – Occluding junctions as novel regulators of tissue mechanics during wound repair. Journal of Cell Biology. ISSN 0021-9525. Vol. 217, N.º 12 (2018), pp. 4267-4283pt_PT
dc.identifier.doi10.1083/jcb.201804048pt_PT
dc.identifier.issn0021-9525
dc.identifier.issn1540-8140
dc.identifier.urihttp://hdl.handle.net/10400.21/9855
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherRockefeller University Presspt_PT
dc.relationPD/BD/106058/2015 - Fundação para a Ciência e a Tecnologiapt_PT
dc.relationTHE ROLE OF SEPTATE JUNCTIONS DURING EPITHELIAL WOUND HEALING IN DROSOPHILA
dc.relationPTDC/FIS-MAC/28146/2017 - Fundação para a Ciência e a Tecnologiapt_PT
dc.relationPTDC/BIA-BID/29709/2017 - Fundação para a Ciência e a Tecnologiapt_PT
dc.relation2007-StG-208631 - European Research Councilpt_PT
dc.relationERC-2015-PoC-713735-EMO DI - European Research Councilpt_PT
dc.relationPIEF-GA-2009-255573 - European Commission Marie Curie Intra-European Fellowshippt_PT
dc.relation30920XM - Partenariat Hubert Curien Pessoa programpt_PT
dc.relation6839 - European Science Foundation (Quantitative Models of Cellular and Developmental Biology Research Networking Program)pt_PT
dc.subjectOccluding junctions (OJs)pt_PT
dc.subjectNovel regulatorspt_PT
dc.subjectTissue mechanicspt_PT
dc.subjectWound repairpt_PT
dc.titleOccluding junctions as novel regulators of tissue mechanics during wound repairpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleTHE ROLE OF SEPTATE JUNCTIONS DURING EPITHELIAL WOUND HEALING IN DROSOPHILA
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F84569%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FFIS%2F00618%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04462%2F2013/PT
oaire.citation.endPage4283pt_PT
oaire.citation.issue12pt_PT
oaire.citation.startPage4267pt_PT
oaire.citation.titleJournal of Cell Biologypt_PT
oaire.citation.volume217pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameCristina de Jesus Carvalho
person.familyNamePatricio
person.familyNamePonte
person.familyNameHeisenberg
person.familyNameAlmeida
person.familyNameAraujo
person.familyNameJacinto
person.givenNameLara
person.givenNamePedro
person.givenNameSusana
person.givenNameCarl-Philipp
person.givenNameLuis
person.givenNameNuno
person.givenNameAntonio
person.identifier94317
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person.identifier.ciencia-idDF15-1DEB-A48C
person.identifier.orcid0000-0001-6350-3279
person.identifier.orcid0000-0002-9050-9956
person.identifier.orcid0000-0002-4297-1265
person.identifier.orcid0000-0002-0912-4566
person.identifier.orcid0000-0002-3344-0366
person.identifier.orcid0000-0002-1677-6060
person.identifier.orcid0000-0002-4193-6089
person.identifier.ridA-2750-2009
person.identifier.ridB-6313-2008
person.identifier.ridF-5729-2013
person.identifier.scopus-author-id56720335000
person.identifier.scopus-author-id13607656900
person.identifier.scopus-author-id55880355900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
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