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Optimized Production of Hydroxamic Acid Derivatives with Antioxidant and Anticholinergic Potential by Immobilized Pseudomonas aeruginosa Cells

dc.contributor.authorBernardo, Marisa
dc.contributor.authorReis, Telma
dc.contributor.authorMinhalma, Miguel
dc.contributor.authorKarmali, Amin
dc.contributor.authorSerralheiro, Maria Luísa
dc.contributor.authorPacheco, Rita
dc.date.accessioned2017-09-28T11:28:53Z
dc.date.available2017-09-28T11:28:53Z
dc.date.issued2017-08
dc.description.abstractIn this study were investigated, the synthesis, acetylcholinesterase inhibition and antioxidant activity of a series of hydroxamic acid derivatives (HAD), with different chemical group characteristics, such as aliphatic (acetohydroxamic acid and butyryl hydroxamic acid), aromatic (benzohydroxamic acid and phenylalanine hydroxamic acid) and amino acid (glycine hydroxamic acid and alanine hydroxamic acid). It was observed that these HAD compounds present very promising activity as acetylcholinesterase (AChE) inhibitors and as antioxidants. The aliphatic HAD demonstrated to have a higher inhibitory activity of AChE than amino acid or aromatic HAD. As for the antioxidant activity, a high antioxidant potential was found for all the compounds with EC50 values ranging from 0.19 µM to 1.65 µM. Aiming these applications, a biocatalysis approach was used to obtain these HADs with optimal reactional conditions. In this study, reverse micelles with immobilized Pseudomonas aeruginosa intact cells containing amidase were used as a biocatalyst to catalyze the acyltransferase reaction of the corresponding substrate amide and hydroxylamine to obtain various HAD and this was achieved for the first time with yields of approximately 100 %.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBERNARDO, Marisa [et al] - Optimized Production of Hydroxamic Acid Derivatives with Antioxidant and Anticholinergic Potential by Immobilized Pseudomonas aeruginosa Cells. American Journal of Microbiology and Biotechnology. ISSN: 2375-3005. Vol. 4, N.º5, (2017), pp. 53-60pt_PT
dc.identifier.doihttp://www.aascit.org/journal/ajmbpt_PT
dc.identifier.issn2375-3005
dc.identifier.urihttp://hdl.handle.net/10400.21/7395
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionfile:///C:/Users/utilizador2/Downloads/9190824%20(1).pdfpt_PT
dc.subjectHydroxamic Acid Derivatives (HAD)pt_PT
dc.subjectAcetylcholinesterase Inhibitorspt_PT
dc.subjectAntioxidant Potentialpt_PT
dc.subjectPseudomonas aeruginosapt_PT
dc.subjectBiocatalysispt_PT
dc.titleOptimized Production of Hydroxamic Acid Derivatives with Antioxidant and Anticholinergic Potential by Immobilized Pseudomonas aeruginosa Cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage60pt_PT
oaire.citation.issue5pt_PT
oaire.citation.startPage53pt_PT
oaire.citation.titleAmerican Journal of Microbiology and Biotechnologypt_PT
oaire.citation.volume4pt_PT
person.familyNameBernardo
person.familyNameMinhalma
person.familyNameKarmali
person.familyNamePacheco
person.givenNameMarisa
person.givenNameMiguel
person.givenNameAmin
person.givenNameRita
person.identifier2161276
person.identifier1022927
person.identifier.ciencia-id5C1D-13E2-201B
person.identifier.ciencia-id9F13-D310-B5A3
person.identifier.orcid0000-0003-2661-5380
person.identifier.orcid0000-0002-6770-194X
person.identifier.orcid0000-0003-0419-401X
person.identifier.orcid0000-0001-5192-3006
person.identifier.ridC-3304-2008
person.identifier.ridE-4787-2011
person.identifier.ridC-3062-2012
person.identifier.scopus-author-id37092511100
person.identifier.scopus-author-id6506304514
person.identifier.scopus-author-id6603778216
person.identifier.scopus-author-id8853708300
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb836e2a5-3cf4-4d3d-9062-61d73a37bd64
relation.isAuthorOfPublication8ed0648a-758d-4b1d-b364-f8ea7195308f
relation.isAuthorOfPublication23975b4b-3234-4139-9675-89ea6f9a2332
relation.isAuthorOfPublicationd6907c0a-3f8f-431c-acf2-0b257692f0d4
relation.isAuthorOfPublication.latestForDiscoveryd6907c0a-3f8f-431c-acf2-0b257692f0d4

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