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Molecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileri

2016-09Journal article Restricted access
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dc.contributor.authorDe Pinho Mixão, V.
dc.contributor.authorMendes, A. M.
dc.contributor.authorMaurício, I. L.
dc.contributor.authorCalado, M. M.
dc.contributor.authorNovo, M. T.
dc.contributor.authorBelo, S.
dc.contributor.authorAlmeida, A. P. G.
dc.date.accessioned2017-08-22T10:41:20Z
dc.date.available2017-08-22T10:41:20Z
dc.date.issued2016-09
dc.description.abstractWolbachia pipientis (Rickettsiales: Rickettsiaceae) protects mosquitoes from infections with arboviruses and parasites. However, the effect of its co-infection on vector competence for Dirofilaria immitis (Spirurida: Onchocercidae) in the wild has not been investigated. This study aimed to screen vectors of D. immitis for wPip, to characterize these, and to investigate a possible association between the occurrence of W. pipientis and that of the nematode. The presence of W. pipientis was assessed in the five mosquito potential vectors of D. immitis in Portugal. Polymerase chain reaction (PCR) products were sequenced, and wPip haplotypes were determined by PCR-restricted fragment length polymorphism (RFLP). Results showed that wPip was detected in 61.5% of Culex pipiens (Diptera: Culicidae) pools and 6.3% of Culex theileri pools. wPip 16s rRNA sequences found in Cx. theileri exactly match those from Cx. pipiens, confirming a mosquito origin, rather than a nematode origin, as some specimens were infected with D. immitis. Only wPip haplotype I was found. No association was found between the presence of wPip and D. immitis in mosquitoes and hence a role for this endosymbiont in influencing vectorial competence is yet to be identified. This study contributes to understanding of wPip distribution in mosquito populations and, to the best of the authors' knowledge, is the first report of natural infections by wPip in Cx. theileri.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationDe Pinho Mixão V, Mendes AM, Maurício IL, Calado MM, Novo MT, Belo S, et al. Molecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileri. Med Vet Entomol. 2016;30(3):301-9.pt_PT
dc.identifier.doi10.1111/mve.12179pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7345
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/mve.12179/abstractpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCulex pipienspt_PT
dc.subjectCulex theileript_PT
dc.subjectDirofilariasispt_PT
dc.subjectMosquitopt_PT
dc.subjectwPippt_PT
dc.subjectPortugalpt_PT
dc.titleMolecular detection of Wolbachia pipientis in natural populations of mosquito vectors of Dirofilaria immitis from continental Portugal: first detection in Culex theileript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage309pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage301pt_PT
oaire.citation.titleMedical and Veterinary Entomologypt_PT
oaire.citation.volume30pt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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