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Fostering protein-calixarene interactions: from molecular recognition to sensing

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dc.contributor.authorPrata, José Virgílio
dc.contributor.authorD. Barata, Patrícia
dc.date.accessioned2017-07-13T11:09:28Z
dc.date.available2017-07-13T11:09:28Z
dc.date.issued2016
dc.description.abstractTwo isomeric bis-calixarene-carbazole conjugates (CCC-1 and CCC-2) endowed with carboxylic acid functions at their lower rims have been found to display a high sensing ability (KSV up to 6 x 10(7) M-1) and selectivity toward cytochrome c, a multi-functional protein, in an aqueous-based medium. After targeting basic amino acid residues on the protein surface residing near the prosthetic heme group through electrostatic and hydrophobic interactions, a rapid photoinduced electron transfer ensues between the integrated transduction element (aryleneethynylene chromophore) of CCCs and the iron-oxidized heme of cytochrome c, enabling direct detection of the protein at nanomolar levels. Our results show that CCCs are capable of efficiently discriminating heme proteins (cytochrome c vs. myoglobin) and non-heme proteins (lysozyme) in an aqueous medium. Studies performed in two solvent systems (organic and aqueous) strongly suggest that in an organic medium a Forster-type resonance energy transfer is responsible for the observed reduction in CCCs emission upon contact with heme proteins while in an aqueous medium a specific photoinduced electron transfer mechanism prevails.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPRATA, José V.; BARATA, Patrícia D. - Fostering protein-calixarene interactions: from molecular recognition to sensing. RSC ADVANCES. ISSN 2046-2069. Vol. 6, N.º 2, (2016), pp. 1659-1669pt_PT
dc.identifier.doi10.1039/c5ra19887pt_PT
dc.identifier.issn2046-2069
dc.identifier.urihttp://hdl.handle.net/10400.21/7270
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://www.rsc.org/suppdata/c5/ra/c5ra19887a/c5ra19887a1.pdfpt_PT
dc.titleFostering protein-calixarene interactions: from molecular recognition to sensingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1669pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage1659pt_PT
oaire.citation.titleRSC Advancespt_PT
oaire.citation.volume6pt_PT
person.familyNamePrata
person.familyNameBarata
person.givenNameJosé Virgílio
person.givenNamePatrícia
person.identifier191765
person.identifier1090399
person.identifier.ciencia-idC11A-9130-8981
person.identifier.ciencia-idC019-9F30-0F99
person.identifier.orcid0000-0002-9068-2847
person.identifier.orcid0000-0001-8274-633X
person.identifier.ridB-7214-2008
person.identifier.scopus-author-id9276778400
person.identifier.scopus-author-id8968446000
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication15e229f3-a619-4931-87eb-799091dc1601
relation.isAuthorOfPublication4ab8a01f-c11d-4b3b-b0cf-b21e5b634170
relation.isAuthorOfPublication.latestForDiscovery4ab8a01f-c11d-4b3b-b0cf-b21e5b634170

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