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Prolonging fixation time of an alternative fixative to formalin for dermatological samples using standard laboratory protocols

2021-03Journal article Restricted access
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dc.contributor.authorSmith, Julie
dc.contributor.authorFaria, Cláudia Sofia
dc.contributor.authorQvist, Camilla Christine
dc.contributor.authorMelchior, Linea C.
dc.contributor.authorLauridsen, Thomas
dc.contributor.authorAntunes, Angélicapt_PT
dc.date.accessioned2020-08-25T14:48:16Z
dc.date.available2024-03-26T01:30:18Z
dc.date.issued2021-03
dc.description.abstractAims: Though formalin remains to be the gold standard fixative in pathology departments, analytical challenges persist for nucleic acid evaluations. In our laboratory, formalin fixation of skin samples, in particular, impairs diagnostic accuracy and demands repetition of biopsies and analytical procedures. PAXgene Tissue Systems may be an alternative; however, according to manufacturer specifications, it only allows fixation for 48 hours before having to add a stabilizer. This may be a challenge in laboratories, which are closed on weekends and bank holidays. Our aim was to validate this alternative fixative for dermatological samples with prolonged fixation times using standard laboratory protocols developed for formalin-fixed specimens. We compared the results with gold standard formalin fixation. Methods: Skin specimens were formalin or PAXgene fixed for either 2 hours, 24 hours, 3 days or 7 days, paraffin-embedded, analyzed, and scored by observers. Results: Generally, formalin outperformed PAXgene fixation in H&E stains and fluorescence in situ hybridization (FISH), but both seem usable for diagnostics. The time of PAXgene fixation did not have an impact on alcian blue-Van Gieson (ABVG), H&E (p=0.48), nor immunohistochemistry (p=0.74). There was a tendency towards best PAXgene performance at 24 hours of fixation for FISH and for DNA integrity analysis 24 hours or 3 days. Conclusions: Prolonging PAXgene fixation time to 3 days before adding stabilizer does not seem to have a major impact on the performance of general diagnostic analysis, but our preliminary results show the optimisation of internal protocols is needed. PAXgene is an expensive alternative and may be confined to some dermatological samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSmith J, Antunes A, Faria CS, Qvist CC, Melchior LC, Lauridsen T. Prolonging fixation time of an alternative fixative to formalin for dermatological samples using standard laboratory protocols. J Clin Pathol. 2021;74(3):149-56.pt_PT
dc.identifier.doi10.1136/jclinpath-2020-206612pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12187
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMJ Publishing Grouppt_PT
dc.relation.publisherversionhttps://jcp.bmj.com/content/74/3/149pt_PT
dc.subjectDiagnostic techniquespt_PT
dc.subjectProcedurespt_PT
dc.subjectImmunohistochemistrypt_PT
dc.subjectMedical laboratory sciencept_PT
dc.subjectMolecular biologypt_PT
dc.subjectSkinpt_PT
dc.titleProlonging fixation time of an alternative fixative to formalin for dermatological samples using standard laboratory protocolspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage156pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage149pt_PT
oaire.citation.titleJournal of Clinical Pathologypt_PT
oaire.citation.volume74pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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