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What sampling method to apply for the occupational exposure assessment to bioburden in Portuguese bakeries?

2018-09Conference object Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorMonteiro, Ana
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorViegas, Susana
dc.date.accessioned2018-11-15T10:39:28Z
dc.date.available2018-11-15T10:39:28Z
dc.date.issued2018-09
dc.descriptionAutoridade para as Condições de Trabalho - Projeto 005DDB/12pt_PT
dc.description.abstractSampling for bioburden, such as fungi and bacteria, in occupational environments, can provide valuable information needed to characterize the exposure conditions, decide whether the contamination signifies a potential hazard to health, and establish the need for control measures. This study intends to suggest the best active sampling approach for the occupational exposure assessment to bioburden in Portuguese bakeries. Twenty air samples were collected through an impaction and impinger methods and two different culture media were used in order to enhance the selectivity for bacterial and fungal populations growth: tryptic soy agar (TSA) supplemented with nystatin (0.2%) used for mesophilic bacterial population and violet red bile agar (VRBA) for bacteria belonging to the Enterobacteriaceae family (e.g. coliforms – Gram-negative bacteria) and malt extract agar (MEA) supplemented with chloramphenicol (0.05%) and dichloran glycerol (DG18) agar-based media were used for fungi. Among the two methods - impaction and impinger - statistically significant differences were observed in the following counts: fungal on MEA (z = -2.721, p = 0.007), fungal on DG18 (z = -4.830, p = 0.000), total bacteria (z= -5.435, p = 0.000) and bacteria Gram – (z = -3.716, p = 0.000). In either case, it has been found that the impaction method detects significantly higher concentrations than the impinger method. Further studies should be performed to propose a more refined protocol to be suggested to exposure assessors that have the bioburden risk characterization as a daily task.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Monteiro A, Carolino E, Viegas S. What sampling method to apply for the occupational exposure assessment to bioburden in Portuguese bakeries? In: 9th International Conference on the Science of Exposure Assessment, Manchester (UK), September 24-26, 2018.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/9040
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationAutoridade para as Condições de Trabalho - Projeto 005DDB/12pt_PT
dc.relation.publisherversionhttps://www.x2018.org/x2018-presentations/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectOccupational exposurept_PT
dc.subjectBioburdenpt_PT
dc.subjectBakerypt_PT
dc.subjectPortugalpt_PT
dc.subjectProjeto 005DDB/12pt_PT
dc.titleWhat sampling method to apply for the occupational exposure assessment to bioburden in Portuguese bakeries?pt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceManchesterpt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscovery77930d39-ed34-44dc-a4a6-9bf833e5e688

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