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Assessment of the microbial contamination of mechanical protection gloves used on waste sorting industry: a contribution for the risk characterization

2020-10Journal article Restricted access
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dc.contributor.authorViegas, Carla
dc.contributor.authorTwarużek, Magdalena
dc.contributor.authorDias, Marta
dc.contributor.authorAlmeida, Beatriz
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorKosicki, Robert
dc.contributor.authorSoszczyńska, Ewelina
dc.contributor.authorGrajewski, Jan
dc.contributor.authorAranha Caetano, Liliana
dc.contributor.authorViegas, Susana
dc.date.accessioned2020-07-28T10:49:12Z
dc.date.available2020-07-28T10:49:12Z
dc.date.issued2020-10
dc.descriptionProject EXPOsE. Project nº 23222 (02/SAICT/2016).pt_PT
dc.descriptionProject Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devices. Project IPL/2018/WasteFRPD_ESTeSL.pt_PT
dc.description.abstractIn Portugal, mechanical protection gloves (MPG) are of mandatory use and during their use sweat is released and, consequently, the humidity of the material increases leading to conditions favorable to the growth of microorganisms. However, no studies have been conducted in MPG to assess the bioburden. This study intended to determine the bioburden present in MPG and their biological effects and to discuss the possibility to use MPG as a passive method to assess occupational exposure to microbial contamination. Fungal burden was characterized through molecular tools for fungal toxigenic species, and antifungal resistance and mycotoxin profiles were determined. Cell viability was determined in swine kidney (SK) monolayer and hepatocellular carcinoma (Hep G2) cell lines. All MPG samples presented Gram-negative bacteria. The fungal contamination ranged from 0 CFU.m−2 in both MEA and DG18, to 5.09 × 106 and 2.75 × 106, and the most common fungi found was Aspergillus spp. (50.46%). Azole resistant Aspergillus sections were found in azole supplemented media. Aspergillus sections (Circumdati, Flavi, Fumigati and Versicolores) were detected by molecular tools in 66 out of 67 samples. The most-reported mycotoxin was mycophenolic acid (89.6%). HepG2 cells appear to be more sensitive to MPG contamination, with high cytotoxicity (IC50 < 0.05 mm2/ml) observed for 18 out of 57 gloves. MPG can be used in passive sampling to assess occupational exposure to bioburden in waste sorting industries and contribute to risk characterization. Some contaminants of MPG had cytotoxic potential and affected the biology of hepatic cells more than renal cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationViegas C, Twarużek M, Dias M, Carolino E, Caetano LA, Viegas S, et al. Assessment of the microbial contamination of mechanical protection gloves used on waste sorting industry: a contribution for the risk characterization. Environ Res. 2020;189:ID109881.pt_PT
dc.identifier.doi10.1016/j.envres.2020.109881pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/12101
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationProject EXPOsEpt_PT
dc.relationProject nº 23222 (02/SAICT/2016)pt_PT
dc.relationProject Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devicespt_PT
dc.relationProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0013935120307763pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectOccupational healthpt_PT
dc.subjectContamination exposurept_PT
dc.subjectWaste sorting industrypt_PT
dc.subjectMechanical protection glovespt_PT
dc.subjectMicrobial contaminationpt_PT
dc.subjectAspergilluspt_PT
dc.subjectMycotoxinspt_PT
dc.subjectSwine kidney monolayer cell linept_PT
dc.subjectHepatocellular carcinoma cell linept_PT
dc.subjectPortugalpt_PT
dc.subjectProject EXPOsEpt_PT
dc.subjectProject nº 23222 (02/SAICT/2016)pt_PT
dc.subjectProject Waste Workers' Exposure to Bioburden through Filtering Respiratory Protective Devicespt_PT
dc.subjectProject IPL/2018/WasteFRPD_ESTeSLpt_PT
dc.titleAssessment of the microbial contamination of mechanical protection gloves used on waste sorting industry: a contribution for the risk characterizationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage109881pt_PT
oaire.citation.titleEnvironmental Researchpt_PT
oaire.citation.volume189pt_PT
person.familyNameViegas
person.familyNameCarolino
person.familyNameAranha Caetano
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameLiliana
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-id9716-9DAC-532A
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1496-2609
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
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