Publication
Environmentally relevant concentrations of Bisphenol A interact with doxorubicin transcriptional effects in human cell lines
dc.contributor.author | Ribeiro, Edna | |
dc.contributor.author | Delgadinho, Mariana | |
dc.contributor.author | Brito, Miguel | |
dc.date.accessioned | 2019-08-30T15:10:23Z | |
dc.date.available | 2019-08-30T15:10:23Z | |
dc.date.issued | 2019-08 | |
dc.description.abstract | The worldwide production of synthetic chemicals, including endocrine disruptor chemicals (EDCs), such as Bisphenol A (BPA) has increased significantly in the last two decades. Human exposure to BPA, particularly through ingestion, is continuous and ubiquitous. Although considered a weak environmental estrogen, BPA can induce divergent biological responses through several signaling pathways, including carcinogenesis in hormone-responsive organs. However, and despite the continuous increase of tumor cell-resistance to therapeutic drugs, such as doxorubicin (DOX), information regarding BPA drug interactions is still scarce, although its potential role in chemo-resistance has been suggested. This study aims to assess the potential interactions between environmentally relevant levels of BPA and DOX at a therapeutic dosage on Hep-2 and MRC-5 cell lines transcriptome. Transcriptional effects in key-player genes for cancer biology, namely c-fos, p21, and bcl-xl, were evaluated through qRT-PCR. The cellular response was analyzed after exposure to BPA, DOX, or co-exposure to both chemicals. Transcriptional analysis showed that BPA exposure induces upregulation of bcl-xl and endorses an antagonistic non-monotonic response on DOX transcriptional effects. Moreover, the BPA interaction with DOX on c-fos and p21 expression emphasize its cellular specificity and divergent effects. Overall, Hep-2 was more susceptible to BPA effects in a dose-dependent manner while MRC-5 transcriptional levels endorsed a non-monotonic response. Our data indicate that BPA environmental exposure may influence chemotherapy outcomes, which emphasize the urgency for a better understanding of BPA interactions with chemotherapeutic agents, in the context of risk assessment. | pt_PT |
dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
dc.identifier.citation | Ribeiro E, Delgadinho M, Brito M. Environmentally relevant concentrations of Bisphenol A interact with doxorubicin transcriptional effects in human cell lines. Toxics. 2019;7(3):43. | pt_PT |
dc.identifier.doi | 10.3390/toxics7030043 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10400.21/10445 | |
dc.language.iso | eng | pt_PT |
dc.peerreviewed | yes | pt_PT |
dc.publisher | MDPI | pt_PT |
dc.relation.publisherversion | https://www.mdpi.com/2305-6304/7/3/43/htm | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | Bisphenol A | pt_PT |
dc.subject | Doxorubicin | pt_PT |
dc.subject | Hep-2 cell line | pt_PT |
dc.subject | MRC-5 cell line | pt_PT |
dc.subject | Drug interaction | pt_PT |
dc.subject | Gene transcription | pt_PT |
dc.title | Environmentally relevant concentrations of Bisphenol A interact with doxorubicin transcriptional effects in human cell lines | pt_PT |
dc.type | journal article | |
dspace.entity.type | Publication | |
oaire.citation.issue | 3 | pt_PT |
oaire.citation.startPage | 43 | pt_PT |
oaire.citation.title | Toxics | pt_PT |
oaire.citation.volume | 7 | pt_PT |
person.familyName | Ribeiro | |
person.familyName | Brito | |
person.givenName | Edna | |
person.givenName | Miguel | |
person.identifier.ciencia-id | C414-CDF2-D35A | |
person.identifier.ciencia-id | 231F-F341-7E93 | |
person.identifier.orcid | 0000-0003-1316-7750 | |
person.identifier.orcid | 0000-0001-6394-658X | |
person.identifier.rid | A-7970-2016 | |
person.identifier.scopus-author-id | 35224551000 | |
rcaap.rights | openAccess | pt_PT |
rcaap.type | article | pt_PT |
relation.isAuthorOfPublication | a571bf34-bcda-49ca-b5cb-4cdecbb3d9c7 | |
relation.isAuthorOfPublication | 4252d8e0-800c-4d67-8b13-0b711d860669 | |
relation.isAuthorOfPublication.latestForDiscovery | a571bf34-bcda-49ca-b5cb-4cdecbb3d9c7 |
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