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The comet assay as a tool in human biomonitoring exposure to anaesthetic gases: a systematic review and meta-analysis

2026-01Artigo científico Acesso embargado
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datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorGajski, Goran
dc.contributor.authorHaveric, Anja
dc.contributor.authorMøller, Peter
dc.contributor.authorAzqueta, Amaya
dc.contributor.authorGiovannelli, Lisa
dc.contributor.authorGerić, Marko
dc.contributor.authorStopper, Helga
dc.contributor.authorBankoglu, Ezgi Eyluel
dc.contributor.authorCollins, Andrew
dc.contributor.authorLadeira, Carina
dc.date.accessioned2026-03-24T09:41:00Z
dc.date.available2026-03-24T09:41:00Z
dc.date.issued2026-01
dc.description.abstractAnaesthetic gases are agents used to induce and maintain general anaesthesia during surgical procedures. Common examples include sevoflurane, isoflurane, and desflurane, which act by depressing the central nervous system to produce unconsciousness and analgesia. These gases are administered through a vaporiser and inhaled via a mask or endotracheal tube. While effective, they can contribute to environmental pollution and increase the risk of occupational exposure. Medical personnel working in operating or post-operative facilities are unavoidably exposed to anaesthetic gases. Several adverse health effects have been associated with anaesthetic gas exposure; therefore, this review aims to summarise findings on DNA strand breaks, assessed by the comet assay in leucocytes of exposed medical workers. Standardised mean differences (SMDs) have been calculated by random effects models. The meta-analysis included 16 studies. Of these, 11 showed statistically significant increased levels of DNA strand breaks, whereas another five studies showed no significant effect. Overall, there is an increased level of DNA strand breaks in exposed subjects in unadjusted analysis (SMD = 1.17, 95 % confidence interval: 0.71, 1.62) as well as analysis adjusted for missing studies by the trim-and-fill method (SMD = 0.53, 95 % confidence interval: −0.14, 1.21). In conclusion, this systematic review and meta-analysis demonstrate that exposure to anaesthetic gases in an occupational setting induces primary DNA damage in human leucocytes, warranting further research to minimise any adverse effects on exposed medical personnel. Besides, the relevance of the use of the comet assay in assessing DNA damage in human biomonitoring studies is proven.eng
dc.description.sponsorshipThis work was supported by the affiliated institutions, European Regional Development Fund project KK.01.1.1.02.0007 (Rec-IMI), the European Union—Next Generation EU 533–03–23–0006 (BioMolTox), and the International Comet Assay Working Group (ICAWG).
dc.identifier.citationGajski G, Haveric A, Møller P, Azqueta A, Giovannelli L, Ladeira C, et al. The comet assay as a tool in human biomonitoring exposure to anaesthetic gases: a systematic review and meta-analysis. Mutat Res Rev Mutat Res. 2026;797:108586.
dc.identifier.doi10.1016/j.mrrev.2026.108586
dc.identifier.issn1383-5742
dc.identifier.urihttp://hdl.handle.net/10400.21/22734
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier BV
dc.relationEuropean Regional Development Fund project KK.01.1.1.02.0007 (Rec-IM)
dc.relationEuropean Union—Next Generation EU 533–03–23–0006 (BioMolTox)
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S1383574226000025
dc.relation.ispartofMutation Research - Reviews in Mutation Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectComet assay
dc.subjectDNA damage
dc.subjectHuman biomonitoring
dc.subjectAnesthetics
dc.subjectOccupational exposure
dc.titleThe comet assay as a tool in human biomonitoring exposure to anaesthetic gases: a systematic review and meta-analysiseng
dc.typejournal article
dcterms.referenceshttps://www.sciencedirect.com/science/article/pii/S1383574226000025#ack0005
dspace.entity.typePublication
oaire.citation.startPage108586
oaire.citation.titleMutation Research - Reviews in Mutation Research
oaire.citation.volume797
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameLadeira
person.givenNameCarina
person.identifier144237
person.identifier.ciencia-id801C-1BBA-1D9E
person.identifier.orcid0000-0001-5588-0074
person.identifier.ridJ-2572-2012
person.identifier.scopus-author-id36463788000
relation.isAuthorOfPublication1aef4b60-4197-436b-84ab-80d31cbaed33
relation.isAuthorOfPublication.latestForDiscovery1aef4b60-4197-436b-84ab-80d31cbaed33

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