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Fungal burden in waste industry: an occupational risk to be solved

dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authorSantos, Mateus dos
dc.contributor.authorCarolino, Elisabete
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorSabito, Raquel
dc.contributor.authorViegas, Susana
dc.date.accessioned2015-08-13T11:31:20Z
dc.date.available2015-08-13T11:31:20Z
dc.date.issued2015-04
dc.description.abstractHigh loads of fungi have been reported in different types of waste management plants. This study intends to assess fungal contamination in one waste-sorting plant before and after cleaning procedures in order to analyze their effectiveness. Air samples of 50 L were collected through an impaction method, while surface samples, taken at the same time, were collected by the swabbing method and subject to further macro- and microscopic observations. In addition, we collected air samples of 250 L using the impinger Coriolis μ air sampler (Bertin Technologies) at 300 L/min airflow rate in order to perform real-time quantitative PCR (qPCR) amplification of genes from specific fungal species, namely Aspergillus fumigatus and Aspergillus flavus complexes, as well as Stachybotrys chartarum species. Fungal quantification in the air ranged from 180 to 5,280 CFU m−3 before cleaning and from 220 to 2,460 CFU m−3 after cleaning procedures. Surfaces presented results that ranged from 29 × 104 to 109 × 104 CFU m−2 before cleaning and from 11 × 104 to 89 × 104 CFU m−2 after cleaning. Statistically significant differences regarding fungal load were not detected between before and after cleaning procedures. Toxigenic strains from A. flavus complex and S. chartarum were not detected by qPCR. Conversely, the A. fumigatus species was successfully detected by qPCR and interestingly it was amplified in two samples where no detection by conventional methods was observed. Overall, these results reveal the inefficacy of the cleaning procedures and that it is important to determine fungal burden in order to carry out risk assessment.por
dc.identifier.citationViegas C, Faria T, Santos M, Carolino E, Gomes AQ, Viegas S, et al. Fungal burden in waste industry: an occupational risk to be solved. Environ Monit Assess. 2015;187(4):199.por
dc.identifier.doi10.1007/s10661-015-4412-y
dc.identifier.urihttp://hdl.handle.net/10400.21/4741
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10661-015-4412-ypor
dc.subjectEnvironmental healthpor
dc.subjectFungal burdenpor
dc.subjectWaste industrypor
dc.subjectOccupational riskpor
dc.subjectAssessmentpor
dc.titleFungal burden in waste industry: an occupational risk to be solvedpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage199por
oaire.citation.titleEnvironmental Monitoring and Assessmentpor
oaire.citation.volume187por
person.familyNameViegas
person.familyNameCarolino
person.familyNameViegas
person.givenNameCarla
person.givenNameElisabete
person.givenNameSusana
person.identifier248817
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id1216-EFA3-1E0F
person.identifier.ciencia-idA919-7318-63DC
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0003-4165-7052
person.identifier.orcid0000-0003-1015-8760
person.identifier.ridB-7217-2013
person.identifier.ridF-1012-2015
person.identifier.ridI-4053-2012
person.identifier.scopus-author-id55443609700
person.identifier.scopus-author-id25821697000
person.identifier.scopus-author-id35270591500
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication77930d39-ed34-44dc-a4a6-9bf833e5e688
relation.isAuthorOfPublication13115332-43f7-4048-a8a5-2f2b855a8c92
relation.isAuthorOfPublication.latestForDiscoveryb5fa5da4-50c3-4b88-ae20-1bc63cb485f7

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