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Biological activity and cellular uptake of [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex

2013-05Journal article Open access
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dc.contributor.authorMorais, Tania S.
dc.contributor.authorSantos, Filipa
dc.contributor.authorCôrte-Real, Leonor
dc.contributor.authorMarques, Fernanda
dc.contributor.authorRobalo, Maria Paula
dc.contributor.authorPaulo J. Amorim Madeira
dc.contributor.authorGarcia, M. Helena
dc.date.accessioned2013-11-05T19:06:04Z
dc.date.available2013-11-05T19:06:04Z
dc.date.issued2013-05
dc.description.abstractAnticancer activity of the new [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] (Me(2)bpy = 4,4'-dimethyl-2,2'-bipyridine) complex was evaluated in vitro against several human cancer cell lines, namely A2780, A2780CisR, HT29, MCF7, MDAMB231 and PC3. Remarkably, the IC50 values, placed in the nanomolar and sub-micromolar range, largely exceeded the activity of cisplatin. Binding to human serum albumin, either HSA (human serum albumin) or HSA(faf) (fatty acid-free human serum albumin) does not affect the complex activity. Fluorescence studies revealed that the present ruthenium complex strongly quench the intrinsic fluorescence of albumin. Cell death by the [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex was reduced in the presence of endocytosis modulators and at low temperature, suggesting an energy-dependent mechanism consistent with endocytosis. On the whole, the biological activity evaluated herein suggests that the complex could be a promising anticancer agent. (C) 2013 Elsevier Inc. All rights reserved.por
dc.identifier.citationMORAIS, Tania S.; SANTOS, Filipa; CORTE-REAL, Leonor; MARQUES, Fernanda; ROBALO, M. Paula; MADEIRA, Paulo J. Amorim; GARCIA, M. Helena - Biological activity and cellular uptake of [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complex. Journal of Inorganic Biochemistry. ISSN 0162-0134. Vol. 122(2013), p. 8-17.por
dc.identifier.issn0162-0134
dc.identifier.other10.1016/j.jinorgbio.2013.01.011
dc.identifier.urihttp://hdl.handle.net/10400.21/2865
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Science INCpor
dc.subjectRutheniumpor
dc.subjectCyclopentadienylpor
dc.subjectCytotoxicitypor
dc.subjectEndocytosis modulatorspor
dc.subjectAlbuminpor
dc.subjectFluorescencepor
dc.subjectHumam-Serum-Albuminpor
dc.subjectCyclopentadienyl Derivative Complexespor
dc.subjectIn-Vitropor
dc.subjectHeteroaromatic Ligandspor
dc.subjectRuthenium Complexespor
dc.subjectArene Complexespor
dc.subjectDNA Interactionpor
dc.subjectPTA Complexespor
dc.subjectBinding-Sitespor
dc.subjectCancerpor
dc.titleBiological activity and cellular uptake of [Ru(eta(5)-C5H5)(PPh3)(Me(2)bpy)][CF3SO3] complexpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceNew Yorkpor
oaire.citation.endPage17por
oaire.citation.startPage8por
oaire.citation.titleJournal of Inorganic Biochemistrypor
oaire.citation.volume122por
person.familyNameCôrte-Real
person.familyNameRobalo
person.familyNameMadeira
person.familyNameAnselmo Viegas Garcia
person.givenNameLeonor
person.givenNameMaria Paula
person.givenNamePaulo
person.givenNameMaria Helena
person.identifierH-2175-­2013
person.identifier.ciencia-idF112-59D7-0984
person.identifier.ciencia-idE11C-A704-9C18
person.identifier.ciencia-id5414-9AEF-4194
person.identifier.orcid0000-0002-4974-1075
person.identifier.orcid0000-0002-8200-6910
person.identifier.orcid0000-0001-9922-7392
person.identifier.orcid0000-0002-4344-2218
person.identifier.ridH-5636-2013
person.identifier.ridB-2002-2012
person.identifier.ridB-8914-2008
person.identifier.scopus-author-id55627880500
person.identifier.scopus-author-id9269662100
person.identifier.scopus-author-id24475068300
person.identifier.scopus-author-id6507884466
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication823a9498-17ab-451f-bb8f-56338643ac0c
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