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A novel flow cytometric protocol for assessment of yeast cell adhesion

dc.contributor.authorSilva-Dias, A.
dc.contributor.authorMiranda, Isabel M.
dc.contributor.authorRocha, R.
dc.contributor.authorMonteiro-Soares, M.
dc.contributor.authorSalvador, Alexandre
dc.contributor.authorRodrigues, Acácio G.
dc.contributor.authorPina-Vaz, Cidália
dc.date.accessioned2013-04-23T14:20:47Z
dc.date.available2013-04-23T14:20:47Z
dc.date.issued2012-03
dc.description.abstractMicrobial adhesion is a field of recognized relevance and, as such, an impressive array of tools has been developed to understand its molecular mechanisms and ultimately for its quantification. Some of the major limitations found within these methodologies concern the incubation time, the small number of cells analyzed, and the operator's subjectivity. To overcome these aspects, we have developed a quantitative method to measure yeast cells' adhesion through flow cytometry. In this methodology, a suspension of yeast cells is mixed with green fluorescent polystyrene microspheres (uncoated or coated with host proteins). Within 2 h, an adhesion profile is obtained based on two parameters: percentage and cells-microsphere population's distribution pattern. This flow cytometry protocol represents a useful tool to quantify yeast adhesion to different substrata in a large scale, providing manifold data in a speedy and informative manner.por
dc.identifier.citationSilva-Dias A, Miranda IM, Rocha R, Monteiro-Soares M, Salvador A, Rodrigues AG, et al. A novel flow cytometric protocol for assessment of yeast cell adhesion. Cytometry A. 2012;81(3):265-70.por
dc.identifier.issn1552-4930
dc.identifier.urihttp://hdl.handle.net/10400.21/2458
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/cyto.a.21170/abstractpor
dc.subjectCandidapor
dc.subjectCandida/physiologypor
dc.subjectCell adhesionpor
dc.subjectFlow cytometry/methodspor
dc.subjectFluorescent dyespor
dc.subjectMicrospherespor
dc.subjectSaccharomyces cerevisiae/physiologypor
dc.subjectCytometrypor
dc.titleA novel flow cytometric protocol for assessment of yeast cell adhesionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage270por
oaire.citation.startPage265por
oaire.citation.titleCytometry Part Apor
oaire.citation.volume81por
rcaap.rightsopenAccesspor
rcaap.typearticlepor

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