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Unraveling the role of TBCCD1 protein on cell size control: the regulation of cytoskeleton dynamics and cell junctions

2016-06Conference object Open access
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dc.contributor.authorCamelo, Carolina
dc.contributor.authorPeneda, Catarina
dc.contributor.authorCoyaud, Étienne
dc.contributor.authorRaught, Brian
dc.contributor.authorCâmara, Ana I.
dc.contributor.authorCarmona, Bruno
dc.contributor.authorPinto, Francisco
dc.contributor.authorNarinho, H. Susana
dc.contributor.authorSoares, Helena
dc.date.accessioned2017-12-04T12:45:39Z
dc.date.available2017-12-04T12:45:39Z
dc.date.issued2016-06
dc.description.abstractDuring their lifetime most cells maintain their size. There is increasing evidence showing that this process may be dynamic and that cells can adapt their size in response to external signals and changes in the environment [1], which strongly suggests that cell size is regulated. Both Hippo and IGF/PI3K/AKT/mTORC1 pathways have been described as being involved in cell size/growth control [1]. Interestingly, these pathways are in a cross-talk with others involved and/or dependent on cellular polarity [2]. Our group characterized a centrosomal protein, TBCCD1 (TBCC domain – containing human protein 1) which, when depleted in human retinal epithelial (RPE–1) cells, leads to an abnormal localization of the centrosome at the cell periphery accompanied by the fragmentation of the Golgi apparatus, resulting in the disruption of the intrinsic cell polarity axis “Nucleus-Centrosome-Golgi Apparatus”. Moreover, TBCCD1 – depleted cells are larger, slower and have a lower efficiency in primary cilia assembly than control cells [3]. We identified the TBCCD1 interactome that showed that most of its partners are involved in cell polarity. Furthermore, most of them participate in the formation/maintenance of cell junctions, which are main regulators of cell polarity in epithelia and are upstream of pathways, like Hippo pathway. We also observed that TBCCD1 overexpression affects tubulin acetylation, which supports our results showing that some of the partners are involved in the regulation of the cytoskeleton dynamics, which may affect cell size. Therefore, it is tempting to hypothesize that the mechanisms involved in the establishment of intrinsic cell polarity may also directly/indirectly participate in the regulation of cell size.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCamelo C, Peneda C, Coyaud E, Raught B, Carmona B, Soares H, et al. Unraveling the role of TBCCD1 protein on cell size control: the regulation of cytoskeleton dynamics and cell junctions. In: CQB Day, Faculdade de Ciências (Lisboa), 28 June 2016.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/7638
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://doczz.net/doc/4429422/cqb-day-2016---universidade-de-lisboapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectTBCCD1 proteinpt_PT
dc.subjectCellular polaritypt_PT
dc.titleUnraveling the role of TBCCD1 protein on cell size control: the regulation of cytoskeleton dynamics and cell junctionspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceLisboapt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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