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DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response

2023-04Journal article Open access
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dc.contributor.authorCamacho, Pedro
dc.contributor.authorRibeiro, Edna
dc.contributor.authorPereira, Bruno
dc.contributor.authorVarandas, Teresa
dc.contributor.authorNascimento, João
dc.contributor.authorHenriques, José
dc.contributor.authorDutra-Medeiros, Marco
dc.contributor.authorDelgadinho, Mariana
dc.contributor.authorOliveira, Ketlyn
dc.contributor.authorSilva, Carina
dc.contributor.authorBrito, Miguel
dc.date.accessioned2023-04-26T10:34:45Z
dc.date.available2023-04-26T10:34:45Z
dc.date.issued2023-04
dc.descriptionThis project was partially supported by an IDI&CA grant IPL/2021/DiffMeDiME_ESTeSL by H&TRC - Health & Technology Research Center, ESTeSL, Saúde, Instituto Politécnico de Lisboa and by Retina Institute of Lisbon (IRL).pt_PT
dc.description.abstractPurpose: DNA methylation is involved in Diabetic Retinopathy progression showing a metabolic memory mechanism. However, the association of DNA methyltransferase with diabetic macular edema is still unknown. We aimed to describe the differences in DNA methyltransferase gene expression in patients with different diabetic macular edema responses. Methods: A total of 27 diabetic patients, aged 59-90 years, were prospectively enrolled in this cross-sectional study. The participants were classified into the control group (CG, n = 11), diabetic macular edema responders (rDME, n = 9), and non-responder diabetic macular edema (nrDME, n = 7) after anti-vascular endothelial growth factor (anti-VEGF) treatment. Only cases with a complete ophthalmological examination, digital 133° color fundus, and SD-OCT assessments were used. After RNA extraction and first-strand cDNA synthesis, quantitative real-time PCR was performed with specific primers on the CFX Connect™ Real-Time PCR Detection System to assess differential transcriptional expression patterns. Results: The DNMT1 gene showed a positive correlation (r = 0.617; p = 0.043) with Best Corrected Visual Acuity (BCVA) in CG, a positive correlation (r = 0.917; p = 0.010) with HbA1c in nrDME and a negative correlation (r = -0.659; p = 0.049) with GCL-IPL thickness in rDME. DNMT3A gene showed a positive correlation (r = -0.890; p = 0.001) with Sub-foveal Choroidal thickness in rDME whereas DNMT3b gene showed a negative correlation (r = -0.815; p = 0.007) with HbA1c and RNFL (r = -0.664; p = 0.026) in CG. Conclusions: Patients with similar metabolic profile risk factors showed associated DNA methyltransferase transcriptional expression patterns differences fitting with the anti-VEGF diabetic macular edema response. Further studies are needed to clarify if these results (1) reflect disease evolution, (2) translate the therapeutic impact, (3) or can help to predict the therapeutic resistance profile.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCamacho P, Ribeiro E, Delgadinho M, Oliveira K, Silva C, Brito M, et al. DNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema response. Eur J Ophthalmol. 2023;33(6):2267-74.pt_PT
dc.identifier.doi10.1177/11206721231171623pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/15938
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSagept_PT
dc.relationIPL/2021/DiffMeDiME_ESTeSLpt_PT
dc.relation.publisherversionhttps://journals.sagepub.com/doi/10.1177/11206721231171623pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDNA methylationpt_PT
dc.subjectDiabetic retinopathypt_PT
dc.subjectDiabetes mellituspt_PT
dc.subjectEpigeneticspt_PT
dc.subjectMacular edemapt_PT
dc.subjectIPL/2021/DiffMeDiME_ESTeSLpt_PT
dc.titleDNA methyltransferase expression (DNMT1, DNMT3a and DNMT3b) as a potential biomarker for anti-VEGF diabetic macular edema responsept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2274pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage112067212311716pt_PT
oaire.citation.startPage2267pt_PT
oaire.citation.titleEuropean Journal of Ophthalmologypt_PT
oaire.citation.volume33pt_PT
person.familyNameCamacho
person.familyNameRibeiro
person.familyNameNeves Delgadinho
person.familyNameSilva
person.familyNameBrito
person.givenNamePedro
person.givenNameEdna
person.givenNameMariana Isabel
person.givenNameCarina
person.givenNameMiguel
person.identifierCAJ-5082-2022
person.identifier.ciencia-id271F-B4E1-014E
person.identifier.ciencia-idC414-CDF2-D35A
person.identifier.ciencia-id231E-02E3-D9A9
person.identifier.ciencia-id2411-5936-0820
person.identifier.ciencia-id231F-F341-7E93
person.identifier.orcid0000-0002-2986-5652
person.identifier.orcid0000-0003-1316-7750
person.identifier.orcid0000-0003-0586-9154
person.identifier.orcid0000-0003-1021-7935
person.identifier.orcid0000-0001-6394-658X
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id55258764900
person.identifier.scopus-author-id35224551000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationc41e9c52-157e-4375-8005-b609cfc374e7
relation.isAuthorOfPublicationa571bf34-bcda-49ca-b5cb-4cdecbb3d9c7
relation.isAuthorOfPublicationca55aab6-9a58-4f79-ab79-20513414099f
relation.isAuthorOfPublication81a5cd80-1982-43ba-bde5-4c43ae0e5234
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relation.isAuthorOfPublication.latestForDiscoverya571bf34-bcda-49ca-b5cb-4cdecbb3d9c7

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