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The nuclear levels of thioredoxin reductase 1, gamma-H2AX, and yap are modulated by primary cilia in response to high glucose levels

2023-07Conference object Open access
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dc.contributor.authorMarques, Rita
dc.contributor.authorPaiva, Marianapt_PT
dc.contributor.authorGinete, Catarina
dc.contributor.authorNolasco, Sofiapt_PT
dc.contributor.authorMarinho, Susana H.pt_PT
dc.contributor.authorVeiga, Luisa
dc.contributor.authorBrito, Miguel
dc.contributor.authorSoares, Helena
dc.contributor.authorCarmona, Bruno
dc.date.accessioned2023-08-01T17:10:02Z
dc.date.available2023-08-01T17:10:02Z
dc.date.issued2023-07
dc.descriptionThis work was funded by Instituto Politécnico de Lisboa IPL/2021/ ObeCil_ESTeSL & IPL/ WintCilGlu_ESTeSL. H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020.pt_PT
dc.description.abstractDiabetes is a condition characterized by impaired regulation of blood glucose levels, leading to various complications such as hypertension, cardiovascular disease, and retinopathy. Diabetic retinopathy (DR), caused by a disrupted retinal blood barrier, is associated with oxidative stress resulting from dysregulated glucose levels in the retina. The primary cilium, an organelle involved in energy balance and glucose homeostasis, has been implicated in the development of various diseases known as ciliopathies, which include overlapping phenotypes such as obesity, diabetes, and retinopathy. This study aims to investigate the impact of high glucose levels on primary cilia assembly in retinal pigment epithelium (RPE-1) cell cultures and explore the role of cilia in the cellular response to high glucose levels. RPE-1 cells were grown in media supplemented with different glucose concentrations (5 mM, 25 mM, and 5 mM glucose + 20 mM mannitol), and cilia assembly was induced before or after glucose supplementation. The results revealed that glucose supplementation did not affect the number of ciliated cells, but cells supplemented with 25 mM glucose exhibited shorter cilia. To understand the role of cilia in response to high glucose levels, the nuclear levels of thioredoxin reductase 1 (TRXR1), a key enzyme involved in combating oxidative stress triggered by hyperglycemia, were evaluated. Additionally, γH2AX, a marker of DNA breaks and cellular senescence, and YAP, a Hippo pathway effector, were examined. It was observed that glucose supplementation, particularly at high levels (25 mM), influenced the nuclear levels of TRXR1, γH2AX, and YAP. Notably, the presence of cilia modulated the cellular response to high glucose levels, modulating the levels of these proteins. These preliminary findings indicate that primary cilia significantly influence the cellular response to high glucose concentrations, which are known to induce oxidative stress and potentially contribute to the development of DR.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarmona B. The nuclear levels of thioredoxin reductase 1, gamma-H2AX, and yap are modulated by primary cilia in response to high glucose levels. In: V H&TRC Bootcamp 2023, Caldas da Rainha, 10 de julho de 2023.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/16360
dc.language.isoengpt_PT
dc.peerreviewednopt_PT
dc.relationIPL/2021/ ObeCil_ESTeSLpt_PT
dc.relationIPL/WintCilGlu_ESTeSLpt_PT
dc.relation.publisherversionhttps://htrcenter.wordpress.com/2023/06/26/v-bootcamp-july-2023/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectObesidadept_PT
dc.subjectCílio primáriopt_PT
dc.subjectGlucosept_PT
dc.subjectIPL/2021/ObeCil_ESTeSLpt_PT
dc.subjectIPL/WintCilGlu_ESTeSLpt_PT
dc.subjectFCT_UIDB/05608/2020pt_PT
dc.subjectFCT_UIDP/05608/2020pt_PT
dc.titleThe nuclear levels of thioredoxin reductase 1, gamma-H2AX, and yap are modulated by primary cilia in response to high glucose levelspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCaldas da Rainhapt_PT
person.familyNameHonrado Ginete
person.familyNameVeiga
person.familyNameBrito
person.familyNameAntunes Soares
person.familyNameSousa Carmona
person.givenNameAna Catarina
person.givenNameLuisa
person.givenNameMiguel
person.givenNameMaria Helena
person.givenNameBruno Filipe
person.identifierG-3065-2010
person.identifier.ciencia-id8715-F62E-1E0F
person.identifier.ciencia-id9413-918D-DB0E
person.identifier.ciencia-id231F-F341-7E93
person.identifier.ciencia-id131B-F0E1-572C
person.identifier.ciencia-id681F-6045-F8C2
person.identifier.orcid0000-0002-2334-782X
person.identifier.orcid0000-0003-1153-8343
person.identifier.orcid0000-0001-6394-658X
person.identifier.orcid0000-0001-6180-7041
person.identifier.orcid0000-0003-0871-9063
person.identifier.ridL-2730-2013
person.identifier.ridA-7970-2016
person.identifier.scopus-author-id8318978600
person.identifier.scopus-author-id35224551000
person.identifier.scopus-author-id55932139400
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationdfb2fbba-17ff-42fb-905a-fcfc8f326e1c
relation.isAuthorOfPublicationaac1914a-275f-4be2-819d-ab41d356b45a
relation.isAuthorOfPublication4252d8e0-800c-4d67-8b13-0b711d860669
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relation.isAuthorOfPublication908e548e-eaac-4485-97c5-fcbd33fe7e5a
relation.isAuthorOfPublication.latestForDiscoveryaac1914a-275f-4be2-819d-ab41d356b45a

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