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Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions

2012-01Journal article Open access
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dc.contributor.authorOliveira, P. de
dc.contributor.authorGomes, Anita Q.
dc.contributor.authorPacheco, T. R.
dc.contributor.authorVitorino de Almeida, V.
dc.contributor.authorSaldanha, C.
dc.contributor.authorCalado, A.
dc.date.accessioned2013-08-21T13:36:03Z
dc.date.available2013-08-21T13:36:03Z
dc.date.issued2012-01
dc.description.abstractAcetylcholine (ACh) has been shown to exert an anti-inflammatory function by down-modulating the expression of pro-inflammatory cytokines. Its availability can be regulated at different levels, namely at its synthesis and degradation steps. Accordingly, the expression of acetylcholinesterase (AChE), the enzyme responsible for ACh hydrolysis, has been observed to be modulated in inflammation. To further address the mechanisms underlying this effect, we aimed here at characterizing AChE expression in distinct cellular types pivotal to the inflammatory response. This study was performed in the human acute leukaemia monocytyc cell line, THP-1, in human monocyte-derived primary macrophages and in human umbilical cord vein endothelial cells (HUVEC). In order to subject these cells to inflammatory conditions, THP-1 and macrophage were treated with lipopolysaccharide (LPS) from E.coli and HUVEC were stimulated with the tumour necrosis factor α (TNF-α). Our results showed that although AChE expression was generally up-regulated at the mRNA level under inflammatory conditions, distinct AChE protein expression profiles were aurprisingly observed among the distinct cellular types studied. Altogether, these results argue for the existence of cell specific mechanisms that regulate the expression of acetylcholinesterase in inflammation.por
dc.identifier.citationOliveira P, Gomes AQ, Pacheco TR, Vitorino de Almeida V, Saldanha C, Calado A. Cell-specific regulation of acetylcholinesterase expression under inflammatory conditions. 2012;51(2):129-37.por
dc.identifier.issn1875-8622
dc.identifier.urihttp://hdl.handle.net/10400.21/2617
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherIOS Presspor
dc.relation.publisherversionhttp://iospress.metapress.com/content/6203r303n18k4330/?genre=article&issn=1386-0291&volume=51&issue=2&spage=129por
dc.subjectAcetylcholinesterasepor
dc.subjectInflammationpor
dc.subjectGene expressionpor
dc.titleCell-specific regulation of acetylcholinesterase expression under inflammatory conditionspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage137por
oaire.citation.startPage129por
oaire.citation.titleClinical Hemorheology and Microcirculationpor
oaire.citation.volume51por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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