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Microbiota assessment in optical shops: an ignored concern to public health

2016-06Conference object Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorFaria, Tiago
dc.contributor.authorPacífico, Cátia
dc.contributor.authorLança, Carla
dc.contributor.authorMonteiro, Ana
dc.contributor.authorCabo Verde, Sandra
dc.date.accessioned2016-06-30T10:48:39Z
dc.date.available2016-06-30T10:48:39Z
dc.date.issued2016-06
dc.description.abstractThe presence of microorganisms in ophthalmic instruments and surfaces can lead to the exposure of patients to several infections. However, there is no information regarding fungal and bacteria contamination in optical shops. This study aims to characterize fungi and bacteria contamination in air and surfaces from 10 optical shops covering also ophthalmic instruments. Air samples were collected through an impaction method onto malt extract agar (MEA) supplemented with chloramphenicol (0.05%) used for fungi and Tryptic Soy Agar (TSA) supplemented with nystatin (0.2%) used for bacteria. Outdoor samples were also performed to be used as reference. Surface and equipment’s swab samples were also collected side-by-side. All the collected samples were incubated at 27ºC for 5 to 7 days (fungi) or at 30º for 7 days (bacteria). Regarding fungal distribution, thirteen different species/genera were found in the air, being the most common Alternaria sp. (62.0%). Eight different species/genera were identified in the surfaces, ranging from 2 to 5x104 CFU/m2, being the most common A. versicolor complex and Penicillium sp. (40.0%). The trial frames were the most contaminated equipment, since 50.0% of the collected samples were with countless colonies. The airborne bacterial population indicated higher concentrations in the contactology office (average: 133 CFU/m3) than in the client’s waiting rooms (average: 126 CFU/m3). The surface samples indicated bacterial concentrations ranging from 2x104 to 1x106 CFU/m2, pointing out the automatic refractometer as the surface with higher bacterial load.pt_PT
dc.identifier.citationViegas C, Faria T, Pacífico C, Lança C, Monteiro A, Cabo Verde S. Microbiota assessment in optical shops: an ignored concern to public health. In: ICOETOX2016 – International Conference on Occupational & Environmental Toxicology, Porto, 21-23 June 2016.pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.21/6269
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relation.publisherversionhttp://www.icoetox2016.com/pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/pt_PT
dc.subjectOccupational exposurept_PT
dc.subjectFungal contaminationpt_PT
dc.subjectBacteria contaminationpt_PT
dc.subjectPublic healthpt_PT
dc.subjectOphthalmic instrumentspt_PT
dc.subjectOphthalmic surfacespt_PT
dc.subjectOptical shopspt_PT
dc.titleMicrobiota assessment in optical shops: an ignored concern to public healthpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
person.familyNameViegas
person.familyNameLança
person.givenNameCarla
person.givenNameCarla
person.identifier.ciencia-idEE1E-C639-D70F
person.identifier.ciencia-id601A-6412-BF2F
person.identifier.orcid0000-0002-1545-6479
person.identifier.orcid0000-0001-9918-787X
person.identifier.ridB-7217-2013
person.identifier.scopus-author-id55443609700
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublicationb5fa5da4-50c3-4b88-ae20-1bc63cb485f7
relation.isAuthorOfPublication0320b455-ee19-4670-8bf2-10dce9de1bec
relation.isAuthorOfPublication.latestForDiscovery0320b455-ee19-4670-8bf2-10dce9de1bec

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